@article{Bushra_2024,

title = {Molecular basis of sepsis: A new insight into the role of mitochondrial DNA as a damage-associated molecular pattern},

author = {Bushra and Shaik Iqbal Ahmed and Safia Begum and Maaria and Mohammed Safwaan Habeeb and Tahmeen Jameel and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/abs/pii/S1567724924001259?via%3Dihub},

doi = {10.1016/j.mito.2024.101967},

issn = {1567-7249},

year  = {2024},

date = {2024-11-01},

urldate = {2024-11-01},

journal = {Mitochondrion},

volume = {79},

issue = {suppl},

pages = {101967},

publisher = {Elsevier BV},

abstract = {Sepsis remains a critical challenge in the field of medicine, claiming countless lives each year. Despite significant advances in medical science, the molecular mechanisms underlying sepsis pathogenesis remain elusive. Understanding molecular sequelae is gaining deeper insights into the roles played by various damage-associated molecular patterns (DAMPs) and pathogen-associated molecular patterns (PAMPs) in disease pathogenesis. Among the known DAMPs, circulating cell-free mitochondrial DNA (mtDNA) garners increasing attention as a key player in the immune response during sepsis and other diseases. Mounting evidence highlights numerous connections between circulating cell-free mtDNA and inflammation, a pivotal state of sepsis, characterized by heightened inflammatory activity. In this review, we aim to provide an overview of the molecular basis of sepsis, particularly emphasizing the role of circulating cell-free mtDNA as a DAMP. We discuss the mechanisms of mtDNA release, its interaction with pattern recognition receptors (PRRs), and the subsequent immunological responses that contribute to sepsis progression. Furthermore, we discuss the forms of cell-free mtDNA; detection techniques of circulating cell-free mtDNA in various biological fluids; and the diagnostic, prognostic, and therapeutic implications offering insights into the potential for innovative interventions in sepsis management.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Basith_2024,

title = {Efficacy of Tru-cut biopsy of the cervical lymphnodes over FNAC for detecting MTB using CBNAAT histopathology and cultures},

author = {Mohammed Abdul Basith and M. Aleemuddin Naveed and Ashfaq Hasan and Fahad Abdullah Bin Breik and Syed Mahmood Ahmed and Shireen Adeeb Mujtaba Ali},

url = {https://journals.lww.com/jdyu/fulltext/2024/13030/efficacy_of_tru_cut_biopsy_of_the_cervical.11.aspx},

doi = {10.4103/jdrysruhs.jdrysruhs_32_24},

issn = {2950-5356},

year  = {2024},

date = {2024-09-27},

urldate = {2024-07-01},

journal = {Journal of Dr. YSR University of Health Sciences},

volume = {13},

issue = {3},

pages = {247–252},

publisher = {Wolters Kluwer - Medknow},

abstract = {Introduction: The present study was carried out to see the usefulness of Tru-cut biopsy over fine needle aspiration (FNA) in diagnoses of tuberculosis (TB) cervical lymphadenopathy and comparing their yields by performing ZN staining, culture, cytology, histopathology, and CBNAAT.

Observation and Results: It was found that the yield of Tru cut was significantly more in ZN staining and histopathology. Even though the yield was more in culture and CBNAAT also, it was not statistically significant. FNA is a popular method which is very widely used, but it has a low yield. From the results, it was inferred that for FNA, the positive rate was 27.6%, 38.4%, 67.7%, and 43% for the four modalities of ZN staining, CBNAAT, cytology, and acid-fast bacilli cultures, respectively. Comparatively, for Tru-cut biopsy, the positive rate was 36.9%, 46.2%, 92.3%, and 47.7%, respectively, in all four modalities. It was noticed that the highest value was recorded for histopathology, followed by culture and CBNAAT for Tru-cut biopsy procedures. Comparatively, FNA showed the highest negative rate for ZN staining and CBNAAT.

Conclusion: The present study also highlights the importance of Tru-cut biopsy over FNA. It is one of the emerging techniques which if used properly could offer a better yield. Further results and conclusions are discussed in the paper.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yeshwanth_2024,

title = {Mitochondrial potential and Nd1 gene expression analysis in platelets for quality assessment},

author = {Asapanna Naga Sai Venkata Yeshwanth and Sara Jabeen and Aleem Ahmed Khan and Nusrath Fathima},

url = {https://www.scholasticopenaccess.org/SCMS/SCMS-02-0043.pdf},

year  = {2024},

date = {2024-07-16},

urldate = {2024-07-16},

journal = {Scholastic Medical Sciences},

volume = {2},

issue = {5},

pages = {1-4},

abstract = {Background: Platelets are the tiniest blood particles, and their shape shows how lively they are. They are largely concerned with hemostasis, or the process of starting blood coagulation. The diverceful activity of platelet and molecules released from platelet storage granules are both necessary for these various activities. Initiating homeostasis, an inflammatory response, and an immune response at the site of injury during the acute phase

of wound healing, as well as encouraging angiogenesis and tissue regeneration during the repair phase, is examples of how these platelet activities interact synergistically. Therefore mitochondria function/quality plays various important roles in several disease processes. Several studies have demonstrated mitochondria dysfunction in various chronic degenerative diseases (ex: cardiovascular diseases; Type 2 diabetes). Hence, there is need for quality assessment platelet mitochondrial DNA for platelet functions. Materials and Methods: Platelets were incubated with Rhodamine 123 exclusive dye to assess the membrane potential. The cytotoxicity of platelets were assed at different dilution point using MTT assay. The activity of platelet mitochondrial (mtDNA) was assessed using ND1 gene. Results: The aggregation of platelets with Rhodamine dye indicates the active state of platelets. The decline of platelets with high concentration MTT assay was observed. The activity of mtDNA in platelets the ND1 gene and internal control 12s gene were expressed at same indicating the active

presence of mtDNA in platelets. Conclusion: The Rhodamine 123 stain with platelet indicates that the presence of mitochondrial membrane potential. The ND1 gene expression indicates the quality of mitochondrial DNA in platelets.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Khan_2023d,

title = {Assessment of soluble thrombomodulin and soluble endoglin as endothelial dysfunction biomarkers in seriously ill surgical septic patients: correlation with organ dysfunction and disease severity},

author = {Mohammed Affan Osman Khan and Tarun Kumar Suvvari and Syed Asif Shah Harooni and Aleem Ahmed Khan and Syyeda Anees and Bushra},

url = {https://link.springer.com/article/10.1007/s00068-023-02369-8},

doi = {10.1007/s00068-023-02369-8},

issn = {1863-9933},

year  = {2023},

date = {2023-09-23},

urldate = {2023-09-01},

journal = {European Journal of Trauma and Emergency Surgery},

publisher = {Springer Science and Business Media LLC},

abstract = {Background: Sepsis, a complex condition characterized by dysregulated immune response and organ dysfunction, is a leading cause of mortality in ICU patients. Current diagnostic and prognostic approaches primarily rely on non-specific biomarkers and illness severity scores, despite early endothelial activation being a key feature of sepsis. This study aimed to evaluate the levels of soluble thrombomodulin and soluble endoglin in seriously ill surgical septic patients and explore their association with organ dysfunction and disease severity.

Methodology: A case control study was conducted from March 2022 to November 2022, involving seriously ill septic surgical patients. Baseline clinical and laboratory data were collected within 24 h of admission to the Surgical Intensive Care Unit. This included information such as age, sex, hemodynamic parameters, blood chemistry, SOFA score, qSOFA score, and APACHE-II score. A proforma was filled out to record these details. The outcome of each patient was noted at the time of discharge.

Results: The study found significantly elevated levels of soluble thrombomodulin and soluble endoglin in seriously ill surgical septic patients. The RTqPCR analysis revealed a positive correlation between soluble thrombomodulin and soluble endoglin levels with the qSOFA score, as well as, there was a positive association between RTqPCR soluble thrombomodulin and the SOFA score. These findings indicate a correlation between these biomarkers and organ dysfunction and disease severity.

Conclusion: The study concludes that elevated levels of soluble thrombomodulin and soluble endoglin can serve as endothelial biomarkers for early diagnosis and prognostication in seriously ill surgical septic patients.},

key = {pmid37741913},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Begum_2023,

title = {Differential expression of Claudin-3 and Claudin-4 in endometriosis},

author = {Safia Begum and Shaik Iqbal Ahmed and Bushra and Smita C. Pawar and Aleem Ahmed Khan},

url = {https://soeagra.com/abr/abrsept2023/29.pdf},

doi = {10.15515/abr.0976-4585.14.5.218227},

issn = {0976-4585},

year  = {2023},

date = {2023-09-01},

urldate = {2023-09-01},

journal = {Advances in Bioresearch},

volume = {14},

issue = {5},

pages = {218-227},

abstract = {Endometriosis is a chronic inflammatory disease that is characterized by presence of endometrial lesions in extra-uterine tissues, affecting ~ 190 million women globally. Our study investigated epithelial mesenchymal transition in different grades of endometriosis by tracking the changes as we move from grade 1 to grade 4. Furthermore we also explored the role of claudin-3 and claudin-4 in different grades of endometriosis. We found decreased expression of claudin-3 and claudin-4 in different grades of endometriosis when compared with the control group. Significant decrease in expression of claudin-3 and claudin-4 was evident in grade 3 and grade 4 endometriosis than in grade 1 and grade 2 endometriosis. Claudin being an integral part of tight junctions is inevitable for maintaining cell-cell integrity and epithelial homeostasis. Undermining the claudin-3 and claudin-4 function is assumed to play an important role in pathogenesis of endometriosis because disruption of tight junctions would set free endometrial cells for migration and invasion. Comprehending the role of claudin-3 and claudin-4 would unveil new insights into underlying molecular mechanisms in endometriosis and it also creates opportunities for designing new therapeutic approaches to treat the disease.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fatima_2023,

title = {In vitro isolation and characterization of multipotent postnatal stem cells from human dental pulp: an approach for regeneration of neural crest tissue},

author = {Nikhat Fatima and Aleem Ahmed Khan and Sandeep Kumar Vishwakarma},

url = {https://journals.lww.com/jpbs/fulltext/2023/15002/in_vitro_isolation_and_characterization_of.61.aspx},

doi = {10.4103/jpbs.jpbs_232_23},

issn = {0976-4879},

year  = {2023},

date = {2023-07-11},

urldate = {2023-07-01},

journal = {Journal of Pharmacy and BioAllied Sciences},

volume = {15},

issue = {Suppl 2},

pages = {S1040-S1042},

publisher = {Medknow},

abstract = {Postnatal dental pulp tissues give the proper justification of the stem cell assimilation and characteristic of the multipotent of the stem cells. Researchers use an in vitro isolation process for clarifying the different stages of staining and cell division. Data collected from various sources helps in understanding how the stem cells help in tissue regeneration. It highlights the immunological phenotypes with the synthesis with cDNA for mentioning molecular immunology. Study also mentions the mitochondrial consistency to measure the potentiality regarding the immunology and the way it differs from 0 to 21 days. Researchers also mention the way for the future development by utilizing the key advantages and definite multipotent of the dental stem cells.},

key = {pmid37693979},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2023,

title = {Simultaneous extraction and quantification of circulating mitochondrial and nuclear DNA using a single plasma sample to predict specific molecular diagnostic implications},

author = {Sandeep Kumar Vishwakarma and Nusrath Fathima and Santosh K. Tiwari and Aleem Ahmed Khan},

url = {https://pdf.sciencedirectassets.com/272218/1-s2.0-S1567724922X00068/1-s2.0-S1567724922001076/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEC4aCXVzLWVhc3QtMSJHMEUCIQCFhJYexwJCvLDwtCzCq5ybzBAkEI5ZvwfXkxvfrboAXgIge2ikPzHMZsp%2Fq2oboo3N%2F%2Fyi%2BakD7EaOFUYh7hTD3j4qswUIJxAFGgwwNTkwMDM1NDY4NjUiDLtcVUv99RPKfnFrxCqQBR5HyFAczXeW8tiWtRyhgIL6Ih9KiRfHqmYhMbnxKoFSLvwgN5gvDMBgX3hA1fvFnoh%2FgIMEpG8P3qeVIT%2BaOmswGOwecRc%2Bi%2F%2Ftgs%2FDXwdckLQY8d3JGsU5LsBg5z0G4%2BIkmMDp7DzcfLR0HHGakJdqheXhgMdJ0MCIPK%2FWJp%2BwIWrnMhzQEs3ckG5JD%2BOkG%2FgyErwbARnPpfh2didcEMk9LwT74iZ%2BgEnutKS2%2BZ7fzXQ9rt6XTja2N6nKinRYbEVKwUZ%2Fd%2FOuf6qTPm8snctNLjQGzGnPQOSagk%2FsEM6T2j3WN%2B1p124GHnT%2Bht6BYtiIHsIxjwyiIhJJ8yGyZWoJ3hccZe%2BMGlyTSvO0UTAEoqMPkF%2FLvFRBCeQVHXwXB5iYvSSKvAOeDWJqa6BcoDLoInBI6y4LOYxh%2Fy8tzQdzF3U0Z%2F5G1FoJQEGbtt%2FgDS5tmrMt3AxvND2OC%2B2jKAbCwQgBRnxYjyRdZxYTtC6UlpNXHlSKHy%2B5F%2F%2BkJfKUQSWZDpzj86HFY6SCg2F6dXb8sh9cT1kfbtLoJFX8Ah7o52Gxa5b3L6cJpQgakBMknq3L9mS3diyWjvbiC1My%2FE4v%2FwEVxZtdcJ%2FyZpK0UVCjYesDhOtdAlSW2jyMvqszvfv8P0fCsDr71mJjACAd9j%2B87Qzlsqtej5KoZwOIEEqjrxusy%2F2SFJY7BC%2F%2B%2BJLWcdCqZPOTiJh5Qi2BBMLolqh66xHHXkfx6D8wzIr0GCiaXZTV%2B1UCcQu%2Fy7nGySOTQU5bfjCFfq25XTp6m7tvfFw2SW3H%2FfbDJMH567jhO6VInbMXZczye2cPH0in6PQFmz7bItDMo%2BK7Py0ntQp%2FL1c%2BOBUhQWfqhOx5n%2BB2DS6aMJ36uagGOrEBbOU5TehdIxyu8Ctrpw4BbLfIyiZb9wkzwFhtiRr1EIx%2B2tfg8gGYY%2BIzNVbtvhLIw39DIbKaIbCNl9xxPor4CC5jJw%2FvAERRhCyPpEo9cIDLgfk7Z7AIXZwLX57YgcNbvulPM40H%2BaYaMzEB%2FNB2KMH5lKFaAm%2BTNKyPaBJe2yCoGzFImiKsH6%2BvugD90qp9xhtRCwV5gTjYtx4EGqXSPYior3J9UQiFpDgRbCiBm8DY&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20230923T071913Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTYRZ7E4GVR%2F20230923%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=0d7a5dc29b4d946a1f68dedf15a00a1a40cdc8500bfe6e952eab9d8e0589ebc4&hash=8a761a892fcbb19735062ffbb5d6348f93bd3af790434f5a79e7ad231b537469&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S1567724922001076&tid=spdf-fbc0216d-dc2a-4ff1-be52-a1f1d7f7b923&sid=d3a802bb9c9bc24c5d8ab168346de759acb2gxrqb&type=client&tsoh=d3d3LnNjaWVuY2VkaXJlY3QuY29t&ua=0f0d5750015655570555&rr=80b104a46beb2e84&cc=in},

doi = {10.1016/j.mito.2022.12.003},

issn = {1567-7249},

year  = {2023},

date = {2023-01-01},

urldate = {2023-01-01},

journal = {Mitochondrion},

volume = {68},

pages = {114-124},

publisher = {Elsevier BV},

abstract = {The magnitude of variations in the level of circulating mitochondrial (cir-mtDNA) and nuclear DNA (cir-ncDNA) in different diseases has indicated the need for investigating a discriminative approach for evaluating their diagnostic significance. This study reports a typical in-house process for extracting both types of cir-DNAs from a single plasma sample and assessed their usefulness in discriminating type 2 diabetes mellitus patients from healthy individuals to eliminate the prevailing dispute about their discriminative role and improve their diagnostic value. This approach offers a more precise and valuable tool for distinguishing the impact of cir-mtDNA from cir-ncDNA in diagnostic implications.},

key = {pmid36509340},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tripura_2022,

title = {Long-term and non-invasive in vivo tracking of DiD dye-labeled human hepatic progenitors in chronic liver disease models},

author = {Chaturvedula Tripura and Srinivas Gunda and Sandeep Kumar Vishwakarma and Avinash Raj Thatipalli and Jedy Jose and Mahesh Kumar Jerald and Aleem Ahmed Khan and Gopal Pande},

url = {https://f6publishing.blob.core.windows.net/73565c43-217d-4006-bcc5-e229b8c8ec9f/WJH-14-1884.pdf},

doi = {10.4254/wjh.v14.i10.1884},

isbn = {1948-5152},

year  = {2022},

date = {2022-10-27},

urldate = {2022-10-27},

journal = {World Journal of Hepatology},

volume = {14},

issue = {10},

pages = {1884-1898},

publisher = {Baishideng Publishing Group Inc.},

abstract = {BACKGROUND: Chronic liver diseases (CLD) are the major public health burden due to the continuous increasing rate of global morbidity and mortality. The inherent limitations of organ transplantation have led to the development of stem cell-based therapy as a supportive and promising therapeutic option. However, identifying the fate of transplanted cells in vivo represents a crucial obstacle. AIM: To evaluate the potential applicability of DiD dye as a cell labeling agent for long-term, and non-invasive in vivo tracking of transplanted cells in the liver. METHODS: Magnetically sorted, epithelial cell adhesion molecule positive (1 × 106 cells/mL) fetal hepatic progenitor cells were labeled with DiD dye and transplanted into the livers of CLD-severe combined immunodeficiency (SCID) mice. Near-infrared (NIR) imaging was performed for in vivo tracking of the DiD-labeled transplanted cells along with colocalization of hepatic markers for up to 80 d. The existence of human cells within mouse livers was identified using Alu polymerase chain reaction and sequencing. RESULTS: NIR fluorescence imaging of CLD-SCID mice showed a positive fluorescence signal of DiD at days 7, 15, 30, 45, 60, and 80 post-transplantation. Furthermore, positive staining of cytokeratin, c-Met, and albumin colocalizing with DiD fluorescence clearly demonstrated that the fluorescent signal of hepatic markers emerged from the DiD-labeled transplanted cells. Recovery of liver function was also observed with serum levels of glutamic-oxaloacetic transaminase, glutamate-pyruvate transaminase, and bilirubin. The detection of human-specific Alu sequence from the transplanted mouse livers provided evidence for the survival of transplanted cells at day 80.

CONCLUSION: DiD-labeling is promising for long-term and non-invasive in vivo cell tracking, and understanding the regenerative mechanisms incurred by the transplanted cells. },

key = {pmid36340748},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fathima_2022c,

title = {Role of cell-free DNA for predicting incidence and outcome of patients with ischemic stroke},

author = {Nusrath Fathima and Sandhya Manorenj and Sandeep Kumar Vishwakarma and Aleem Ahmed Khan},

url = {https://f6publishing.blob.core.windows.net/0bb64623-48a1-4d1c-9e53-15650da40f4a/WJN-8-1.pdf},

doi = {10.5316/wjn.v8.i1.1},

issn = {2218-6212},

year  = {2022},

date = {2022-08-30},

urldate = {2022-08-01},

journal = {World Journal of Neurology},

volume = {8},

issue = {1},

pages = {1-9},

publisher = {Baishideng Publishing Group Inc.},

abstract = {Early diagnosis and prognosis of ischemic stroke remains a critical challenge in clinical settings. A blood biomarker can be a promising quantitative tool to represent the clinical manifestations in ischemic stroke. Cell-free DNA (cfDNA) has recently turned out to be a popular circulating biomarker due to its potential relevance for diagnostic applications in a variety of disorders. Despite bright outlook of cfDNA in clinical applications, very less is known about its origin, composition, or function. Several recent studies have identified cell-derived mitochondrial components including mitochondrial DNA (mtDNA) in the extracellular spaces including blood and cerebrospinal fluid. However, the time course of alterations in plasma mtDNA concentrations in patients after an ischemic stroke is poorly understood. DNA is thought to be freed into the plasma shortly after the commencement of an ischemic stroke and then gradually decreased. However, the importance of cell-free mtDNA (cf-mtDNA) in ischemic stroke is still unknown. This review summarizes about the utility of biomarkers which has been standardized in clinical settings and role of cfDNA including cf-mtDNA as a non-invasive potential biomarker of ischemic stroke.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fathima_2022b,

title = {Cell-free mitochondrial DNA quantification in ischemic stroke patients for non-invasive and real-time monitoring of disease status},

author = {Nusrath Fathima and Sandhya Manorenj and Sandeep Kumar Vishwakarma and Aleem Ahmed Khan},

url = {https://f6publishing.blob.core.windows.net/7f307e4a-b015-447a-a322-f1d12b1e8878/WJTM-10-14.pdf},

doi = {10.5528/wjtm.v10.i2.14},

issn = {2220-6132},

year  = {2022},

date = {2022-08-06},

urldate = {2022-08-06},

journal = {World Journal of Translational Medicine},

volume = {10},

issue = {2},

pages = {14-28},

publisher = {Baishideng Publishing Group Inc.},

abstract = {BACKGROUND: Acute ischemic stroke (AIS) is one of the major causes of the continuous increasing rate of global mortality due to the lack of timely diagnosis, prognosis, and management. This study provides a primitive platform for non-invasive and cost-effective diagnosis and prognosis of patients with AIS using circulating cell-free mitochondrial DNA (cf-mtDNA) quantification and validation. AIM: To evaluate the role of cf-mtDNA as s non-invasive, and affordable tool for real-time monitoring and prognosticating AIS patients at disease onset and during treatment. METHODS: This study enrolled 88 participants including 44 patients with AIS and 44 healthy controls with almost similar mean age group at stroke onset, and at 24 h and 72 h of treatment. Peripheral blood samples were collected from each study participant and plasma was separated using centrifugation. The cf-mtDNA concentration was quantified using nanodrop reading and validated through real-time quantitative polymerase chain reaction (RT-qPCR) of NADH-ubiquinone oxidoreductase chain 1 (ND1) relative transcript expression levels. RESULTS: Comparative analysis of cf-mtDNA concentration in patients at disease onset showed significantly increased levels compared to control individuals for both nanodrop reading, as well as ND1 relative expression levels (P < 0.0001). Intergroup analysis of cf-mtDNA concentration using nanodrop showed significantly reduced levels in patients at 72 h of treatment compared to onset (P < 0.01). However, RT-qPCR analysis showed a significant reduction at 24 h and 72 h of treatment compared to the disease onset (P < 0.001). The sensitivity and specificity were relatively higher for RT-qPCR than nanodrop-based cf-mtDNA quantification. Correlation analysis of both cf-mtDNA concentration as well as ND1 relative expression with National Institute of Health Stroke Scale score at baseline showed a positive trend. CONCLUSION: In summary, quantitative estimation of highly pure cf-mtDNA provides a simple, highly sensitive and specific, non-invasive, and affordable approach for real-time monitoring and prognosticating AIS patients at onset and during treatment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ali_2022c,

title = {Role of circulatory miRNA-21 and associated signaling pathways in the pathogenesis of pulmonary fibrosis among individuals recovered after COVID-19 infection},

author = {Mohammad Ali and Fahad Abdullah Bin Breik and M. Aleemuddin Naveed and Syed Mahmood Ahmed and Aleem Ahmed Khan and Ashfaq Hasan},

url = {https://pdf.sciencedirectassets.com/781323/1-s2.0-S2773044122X00041/1-s2.0-S2773044122000675/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEGEaCXVzLWVhc3QtMSJGMEQCIE0LJ%2BCvVLB86Touyqcy2AguFweYOYjtUTEMTkViqBbbAiAI%2BAvwB315j9dC9Se4lbm9nDydykFzM1oYJsMEVqzKmCqyBQhaEAUaDDA1OTAwMzU0Njg2NSIMq8cixabBNQ%2FFlP7RKo8F4tHFQ2XCEX%2BnNUv%2Fo5kJzDriDFR%2B4esGfpxvWYZe2iJLDcqko4%2FKj6UJNujCCeczxRju1D5fVB%2BeBslzhdQvE7hnh%2F%2FnzV7nqibmZXBDBzsscCcFaUKkvp6kTveTxyA2rUH4diDkXnrrhgfmOX2eisr7Qhmd%2F0AcaREv%2BO%2FmbdqUbKMsbAkTgrzYw8Yx9qK%2BgJYzk19T300hlFdoGGGafOWk8DK9wuY5JSMb96a1S%2Bm1kTBHrmHg7dvRgmq2I1GbV2y10AY3eLayG%2FhLZNqxy%2FswXa16YVvtYJ6UG8o998uMZe7Zxu%2FLw73%2Fnjd7E0un%2FhMbpOan6kbXc9KEG%2BqVvtXV2NXKxAZBhIwHXMsDyyt16dwdwcE5f9pLP7vBhiEuK4SbFC0Xo1t4sc2wKNY3uCh0GPpTDa%2FTLWgyY0sPMImc%2FyZ8RjKKL4hrQkmVoNqFqLgdsOPVsqoMxmYbErD0EbMwwVf%2FRBQwSeRKi0Y3CfzAMqOnGUzrghoR9Yha2MG4CpBAHpTaFLIeiuETnxdr7GWzvsEOCqDwtMmoYPKXIEe0aKp%2Fk96I5JniIHBdwlx4OwzzPORgC6qdJ195O9b%2FFvA9zGGsLaNfVhKe3oHRDYapPDQBSvrsgZ7dLldkHOhE0yGQm573V51HZu1i4XdNOLoUje5EzAtEmJe3ALac9yxDO2yu1gI5ygLizO7QtWowln2DDIEwZgfsJguAfo6%2B5YPvapbKcKLXEcCWPik3KBMgkNKvboCwdXor52nrYcUmbVLGGyqbzIMTvWglr5840F4sxHkWbJv%2FBWjbAwTfUtiRfJBEW76e4GNtFK%2FbS7ttN5oHd2D4a%2FjewMBmD5Zb38tmdBC4yNt%2FcndcejUPzTDjk8WoBjqyAdtvZMDfupHhuUIvK8v%2BtQIPbtzLcwwqA8TyxzrCBPad5FAsv%2F3jjLRZkjVmqHwehp4d8QPgKrJYWEfRm8E8Vnuv2c7WxFhoBtYlIoy6CaA3lgtqtbI6j%2F%2FLQ1TZqhk2g5HhF3CyYAU%2FVADQIEPLQ1M6S%2FHxWtEMDZFbrx17lQO1IkEiGEH3k9wxJ11A4Diy3Ny8U3Cvjz1nYvoebZtaoSg%2BCgQtb5jjGEjMJAf7iA5s0MI%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20230925T094841Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTYTGSOBQO3%2F20230925%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=aa7b878d5e9909131750691259b7560c6773fade490297b8b03355bffa840587&hash=ec409bf344f4e3f2a9188514ea2f0cce6751ec7906e16403ebe1144637fa97b4&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S2773044122000675&tid=spdf-d9142a09-f0a7-44e7-aade-22a3c5a32970&sid=7685485b9275d2467038aec-c7fdaf004220gxrqb&type=client&tsoh=d3d3LnNjaWVuY2VkaXJlY3QuY29t&ua=0f0d5750005550025154&rr=80c25a55481a2e84&cc=in},

doi = {10.1016/j.humgen.2022.201093},

issn = {2773-0441},

year  = {2022},

date = {2022-07-28},

urldate = {2022-07-28},

journal = {Human Gene},

volume = {34},

pages = {201093},

publisher = {Elsevier BV},

abstract = {Introduction: Currently pulmonary fibrosis in post-COVID individuals represents a crucial milieu of investigation due to long-term associated complications and worse clinical outcome. Lack of studies in Indian population confers a crucial need for elucidating possible targets and mechanisms to explore better management and outcome. Hence, this study aimed to explore the role of circulating miRNA-21 in patients from South India after COVID-19 recovery, while targeting TGF-β signaling pathway involved in the development of pulmonary fibrosis. Methods: This prospective, single centre, hospital-based study enrolled a total of 50 participants in the age group of 50 to 60 years including 25 non-infected controls and 25 patients who were recovered after 3–6 months of COVID-19 infection and presented radiological pulmonary abnormalities. Quantification of miRNA-21 and selected gene transcripts (TGF-β, Col1A2, Col3A1, and α-SMA) was performed in plasma samples of both patients and controls. Results: Significantly increased expression levels of miRNA-21 was observed in patient samples compared to controls (4.50 ± 1.03 vs 12.60 ± 3.52, p < 0.0001) with 72.10% sensitivity and 80.10% specificity. Further, significantly increased levels of central fibrosis regulatory gene transcript TGF-β (0.56 ± 0.27 vs 1.83 ± 0.98), two crucial collagen transcripts Col1A2 (0.62 ± 0.19 vs 1.56 ± 1.00) and Col3A1 (0.61 ± 0.27 vs 1.54 ± 0.89), and α-SMA (0.46 ± 0.17 vs 1.20 ± 0.78) was observed in patients compared to controls. Western-blot analysis also showed almost similar observations at proteins levels. Conclusion: Circulating miRNA-21 may provide crucial insights for elucidating TGF-β mediated pulmonary remodeling involved in the fibrosis development and achieve better clinical outcome for post-COVID patients after recovery, in real-time with high diagnostic accuracy.},

key = {pmid37521444},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2022,

title = {A comprehensive stemness gene expression analysis signifies the role of ABC transporters and molecular chaperons in determining the fate of human neural precursor cells},

author = {Sandeep Kumar Vishwakarma and Avinash Bardia and Syeda Sumaiya Bibi Fathima and Syed Ameer Basha Paspala and Aleem Ahmed Khan},

url = {https://www.neurologyindia.com/temp/ni7031102-2035976_053919.pdf},

doi = {10.4103/0028-3886.349631},

issn = {0028-3886},

year  = {2022},

date = {2022-07-01},

urldate = {2022-07-01},

journal = {Neurology India},

volume = {70},

issue = {3},

pages = {1102-1111},

publisher = {Medknow},

abstract = {Background and Aim: The major aim of this study was to identify the most common stemness genes across different stem cell types and further validate them in human fetal subventricular zone-derived primary and cultured neural precursor cells (NPCs). This study involved the use of a unique method of stemness meta-analysis (SMA) for investigating comprehensive upregulation and downregulation of differentially expressed genes (DEGs) among different stem cell populations. Materials and Methods: A total of 55 mouse and human data sets targeting crucial genes identified in seven different types of stem cells population were screened and subjected to independent DEGs analysis using SMA. Identified 30 meta-gene signatures were subjected to functional enrichment analysis based on their biological processes and molecular functions. Validation of enriched meta-gene signatures was performed using RT-qPCR. Cellular localization of ABCB1 and ABCG2 was identified using immunofluorescence staining, whereas functional assessment was performed using western-blot. Results: SMA analysis revealed that among 52 commonly expressed genes, 30 genes were either upregulated or downregulated in at least two stem cell populations. Further gene enrichment analysis showed nine genes (ABCB1, ABCG2, HSPA4, HSPA9, HSPA14, Nestin, Sox-2, Oct-4, and Notch-2) with the highest combined scores among 30 meta-gene signatures. RT-qPCR demonstrated that all the enriched gene signatures were significantly upregulated in primary NPCs and further downregulated during NPCs lineage differentiation in culture except HSPA4, HSPA9, and HSPA14 gene transcripts. 

Conclusions: The stemness meta-gene signatures were abundantly expressed in human NPCs population which categorically suggest the involvement of these genes/pathways in pluripotency maintenance and molecular switches for lineage differentiation while HSP-70 had a neuroprotective effect.},

key = {pmid35864646},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Prasad_2022,

title = {Association of angiotensin II type 1 receptor (AT1R) gene polymorphism with angiotensin II serum levels in patients with essential hypertension},

author = {M. Prasad and D. Rajarajeswari and K. Ramlingam and R. Viswakumar and B. Suneel and Jyothi Conjeevaram and P. Aruna and Nusrath Fathima and Sandeep Kumar Vishwakarma and Aleem Ahmed Khan},

url = {https://link.springer.com/article/10.1007/s12291-022-01041-z},

doi = {10.1007/s12291-022-01041-z},

issn = {0970-1915},

year  = {2022},

date = {2022-04-30},

urldate = {2022-04-30},

journal = {Indian Journal of Clinical Biochemistry},

volume = {38},

issue = {1},

pages = {110-119},

publisher = {Springer Science and Business Media LLC},

abstract = {Essential hypertension (EH) is a multifactorial, polygenic condition, and is one of the most important comorbidities that contributes to stroke, myocardial infarction, cardiac failure, and renal failure. The continuous increasing rate of morbidity and mortality associated with EH presents an unmet need of population-based studies to explore pathophysiology as well as newer strategies for better diagnosis, prognosis and treatment. This study aimed to determine genotype and allele frequencies of A1166C polymorphism of AT1R gene in Indian patients with EH and correlated with serum levels of Angiotensin II. A total of 200 patients with EH and 200 age- and gender-matched control individuals were included in this study from the General Medicine Department Outpatient at Narayana Medical College and Hospital, Nellore, Andhra Pradesh, India. Patients with systolic blood pressure (SBP) ≥ 140 mmHg and/or diastolic blood pressure (DBP) ≥ 90 mmHg were considered as hypertensive. The findings of this study revealed significantly increased risk of C/A heterozygote and allele C in both men and women. Moreover, both men and women patients with EH showed higher serum levels of Angiotensin II with C/A as well as AA genotypes. These findings indicate a significant association of 1166 C/A polymorphism of the AT1R gene with increased risk of hypertension in Indian population.},

key = {pmid36684488},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sreerambhatla_2022,

title = {Contribution of genetic variants of DNA repair genes XRCC1 and APE1 in pediatric patients with food and air born allergen asthma},

author = {Nandita Sreerambhatla and Uppin Narayan Reddy and Shaik Iqbal Ahmed and Nusrath Fathima and Aleem Ahmed Khan},

url = {https://www.worldwidejournals.com/indian-journal-of-applied-research-(IJAR)/fileview/contribution-of-genetic-variants-of-dna-repair-genes-xrcc1-and-ape1-in-pediatric-patients-with-food-and-air-born-allergen-asthma_February_2022_8241044964_1206692.pdf},

doi = {10.36106/ijar},

issn = {2249-555X},

year  = {2022},

date = {2022-02-28},

urldate = {2022-02-28},

journal = {Indian Journal of Applied Research},

volume = {12},

issue = {2},

pages = {45-47},

abstract = {INTRODUCTION: Allergic asthma in children occurs in response to an allergic trigger major. In the pathophysiology of asthma, genetic variations in many loci and genes play a crucial role. Genes of DNA repair pathways have been well characterized in association with clinical pathologies of different forms of allergic asthma with conicting outcomes. This study reports the role of single nucleotide polymorphisms (SNPs) in X-ray cross-complementing group 1 (XRCC1) and apurinic/apyrimidinic endonuclease 1 (APE1)polymorphisms in pediatric patients with food and air born allergen asthma. METHODS: 125 pediatric asthma patients and age and gender matched 164 controls were enrolled. Blood samples were collected after conrming the food and air allergen related asthma. Genomic DNA was isolated from whole blood and genotyping was done for XRCC1 Arg399Gln and APE1 Asp148Glu using ARMS-PCR. RESULTS: The frequency of genotype Arg399Gln (heterozygous) of XRCC1 gene was signicantly higher in patients with allergic asthma than the controls (odds ratio [OR] 2.75; 95% condence interval [CI] 1.69 -4.47; p=0.006). 'A' allele of XRCC1 gene was found to be predominant in pediatric asthma group compared to controls. Similarly the genotype TG frequency of APE1 Asp148Glu showed statistically signicant change in allergic asthma patients compare to controls (OR 2.93; 95% CI 1.77-4.85; p<0.001). 'G' allele of APE1 gene was found predominantly in pediatric asthma group compared to controls. CONCLUSION: Polymorphisms in XRCC1 Arg399Gln and APE1 Asp148Glu signicantly increased the risk of allergic asthma in pediatric patients.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fathima_2022,

title = {Autoimmune thyroid patients with CTLA-4 (+49A/G) GG/AG genotypes have high seropositivity to thyroid peroxidase than thyroglobulin},

author = {Nusrath Fathima and Qursheed Sultana and Syyeda Anees and Kaleem Ullah and Vitaly Ryu and Aleem Ahmed Khan and Mohammed Ishaq},

url = {https://www.sciencedirect.com/science/article/abs/pii/S2214540022000019?via%3Dihub},

doi = {10.1016/j.mgene.2022.101010},

issn = {2773-0441},

year  = {2022},

date = {2022-02-01},

urldate = {2022-02-01},

journal = {Meta Gene},

volume = {31},

issue = {suppl C},

pages = {101010},

publisher = {Elsevier BV},

abstract = {Despite increasing burden of autoimmune thyroid disease (AITD) in India, there is paucity of data for the genetic susceptibility in Indian population which represents an important aspect to uncover the genetic factors for the cause or progression of different forms of AITD. It is known that production of auto-antibodies to thyroglobulin (TG) and thyroid peroxidase (TPO) is a characteristic feature of AITD. Production of high titres of auto-antibodies against TG and TPO is one the hallmarks of AITD that often precedes the development of clinical disease. The gene coding for cytotoxic T lymphocyte antigen-4 (CTLA-4) is considered an important candidate gene for susceptibility to AITD and also determines seropositivity. Earlier studies have demonstrated that polymorphic variants in CTLA-4 gene are linked to determine seropositivity. Hence, this study investigates a relationship between CTLA-4 (+49A/G) genotypes and seropositivity to anti-TPO and anti-TG antibodies in AITD patients. A total of 85 Indian participants were included and genotyped for CTLA-4 (+49A/G) single nucleotide polymorphism and further analyzed for seropositivity of TPO and TG auto-antibodies in mean age group of 35.76 ± 12.42 years of both the genders. The TPO seropositivity was reported higher in G allele containing genotypes GG and AG (66.66% and 58%, respectively) compared to AA (44.8%) genotype. Whereas, TG auto-antibodies showed highest seropositivity for genotype AA (55.17%) compared to AG (36%) and GG (16.6%). Higher median titre values were observed for TPO antibodies with GG genotype (1587.461 U/mL). In contrast to this, higher titres of auto-antibodies to TG were observed for individuals with AA genotype (520.746 U/mL). In conclusion, this study demonstrates a relationship between CTLA4 (+49A/G) genotype and seropositivity to thyroid auto antigens TPO and TG in Indian patients with clinical manifestation of Hashimoto's thyroiditis and Graves' disease, two different forms of AITD.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Lakkireddy_2021,

title = {Biofabrication of allogenic bone grafts using cellularized amniotic scaffolds for application in efficient bone healing},

author = {Chandrakala Lakkireddy and Sandeep Kumar Vishwakarma and Avinash Bardia and Nagarapu Raju and Shaik Iqbal and Syeda Maliha Fathima and Sandhya Annamaneni and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0040816621001476?via%3Dihub},

doi = {10.1016/j.tice.2021.101631},

issn = {0040-8166},

year  = {2021},

date = {2021-12-31},

urldate = {2021-12-31},

journal = {Tissue and Cell},

volume = {73},

pages = {101631},

abstract = {Introduction: The reconstruction/regeneration of human bone injuries/defects represents a crucial challenge due to the lack of suitable bio/immune compatible and implantable biological grafts. The available strategies represent implications of several types of grafting materials in the form of metals, synthetic, and various kinds of biological scaffolds; however, the lack of appropriate biological components required for activating and enhancing repair mechanisms at the lesion-site limits their wider applicability. Methods: In this study, a unique approach for generating human osteogenic implantable grafts was developed using biofabrication technology. Using a gradient change of detergents and continuous agitation, developed a unique technique to generate completely cell-free amnion and chorion scaffolds. The absence of cellular components and integrity of biological and mechanical cues within decellularized human amnion (D-HAM) and chorion (D-HCM) were evaluated and compared with fresh membranes. Allogenic bone grafts were prepared through induction of human mesenchymal stem cells (hMSCs) into osteogenic cells on D-HAM and D-HCM and evaluated for their comparative behavior at the cellular, histological and molecular levels. Results: The common decellularization process resulted in an efficient way to generate D-HAM and D-HCM while retaining their intact gross-anatomical architecture, surface morphology, extracellular matrix components, and mechanical properties. Both these scaffolds supported better growth of human umbilical cord blood derived MSCs as well as osteogenic differentiation. Comparative investigation revealed better growth rate and differentiation on D-HCM compared to D-HAM and control conditions. Conclusion: D-HCM could be used as a better choice for producing suitable allogenic bone grafts for efficient bone healing applications. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Lakkireddy_2021,

title = {Fabrication of decellularized amnion and chorion scaffolds to develop bioengineered cell-laden constructs},

author = {Chandrakala Lakkireddy and Sandeep Kumar Vishwakarma and Nagarapu Raju and Shaik Iqbal Ahmed and Avinash Bardia and Mazharuddin Ali Khan and Sandhya Annamaneni and Aleem Ahmed Khan},

url = {https://link.springer.com/article/10.1007/s12195-021-00707-7},

doi = {10.1007/s12195-021-00707-7},

issn = {1865-5025},

year  = {2021},

date = {2021-09-24},

urldate = {2021-09-01},

journal = {Cellular and Molecular Bioengineering},

volume = {15},

issue = {1},

pages = {137-150},

publisher = {Springer Science and Business Media LLC},

abstract = {Introduction: Human mesenchymal stem cells (hMSCs) holds great promise for managing several clinical conditions. However, the low engraftment efficiency and obscurity to harvest these cells without compromising the cellular viability, structural and functional properties from the culture niche still remain major obstacles for preparing intact regenerative constructs. Although few studies have demonstrate different methods for generating cell-liberated amniotic scaffolds, a common method for producing completely cell-liberated amnion (D-HAM) and chorion (D-HCM) scaffolds and their cytocompatibility with hMSCs yet to be demonstrated. Methods: A common process was developed for preparing D-HAM and D-HCM scaffolds for assessing hMSCs engraftment efficiency, proliferation and molecular shifts to generate cell-laden biological discs. The structural and functional integrity of D-HAM and D-HCM was evaluated using different parameters. The compatibility and proliferation efficiency of hMSCs with D-HAM and D-HCM was evaluated. Results: 

Histological analysis revealed completely nucleic acid-free D-HAM and D-HCM scaffolds with intact extracellular matrix, mechanical and biological properties almost similar to the native membranes. Human MSCs were able to adhere and engraft on D-HCM better than D-HAM and expanded faster. Ultrastructural observations, crystal violet staining and expression studies showed better structural and functional integrity of hMSCs on D-HCM than D-HAM and control conditions. Conclusion: A common, simple and reliable process of decellularization can generate large number of cell-liberated amniotic scaffolds in lesser time. D-HCM has better efficiency for hMSCs engraftment and proliferation and can be utilized for preparing suitable cell-laden constructs for tissue engineering applications.},

key = {pmid35096189},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Khatoon_2021,

title = {Serum calcitonin gene related peptide (CGRP) levels in migraine: a study on its clinical correlation and diagnostic efficacy},

author = {Sofia Khatoon and Noorunissa Begum and Hafeeza Sultana and Maryam Rashed and Mohammed Zoheb and Aleem Ahmed Khan and Sara Shreen and Syed Iqbal Ahmed},

url = {https://rjn.com.ro/articles/2021.3/RJN_2021_3_Art-13.pdf},

doi = {10.37897/RJN.2021.3.13},

issn = {1843-8148},

year  = {2021},

date = {2021-08-10},

urldate = {2021-08-10},

journal = {Romanian Journal of Neurology},

volume = {20},

issue = {3},

pages = {342-348},

abstract = {Migraine is a primary headache disorder marked by recurrent unilateral headache episodes. Calcitonin gene related peptide (CGRP) plays major role in migraine pathophysiology. CGRP is multifunctional, and its vasodilating activity within the central and peripheral blood vessels is one in all its primary functions.The intention is to prove serum calcitonin gene related peptide (CGRP) as an early diagnostic tool for migraine and the novelty is to correlate it with characteristics of migraine so that it helps in early initiation of treatment.

Methods. 100 subjects including 90 patients with migraine and 10 with non-headache (NH) age-matched controls were prospectively recruited in our current study. The subjects were aged from 15- 50 years. The clinical assessment was made every month for the three months after the start of therapy. The subjects were compared based on the serum CGRP values. Serum CGRP concentrations were measured by using CGRP ELISA kit. Results. Out of total subjects selected, the maximum (23.3%) subjects were between age 26 and 30 years and least effected age group was 46-50 years (4.44%). Females’ predominance with 82% than males with 18%.Stress was major trigger occurring in 57% of cases. Throbbing pain with elevated CGRP levels 130.44±114.22 and p value (p = 0.01). The average CGRP levels was higher in test group 149.00±93.86 compared to control 61.30±24.37 with p value (p = 0.02). Conclusions. The serum CGRP levels were statistically more in migraine patients correlated with characteristics like throbbing type of pain, stress and inadequate sleep. Hence, the serum CGRP levels estimation can be considered as a diagnostic tool for migraine when the clinical character’s over lap or early in the course of migraine when all criteria for diagnosis are not yet fulfilled.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Begum_2021b,

title = {Prevalence and comprehensive insights into molecular mechanisms in Helicobacter pylori pathogenesis},

author = {Nazima Begum and Syed Mohammed Akbar Hassan and Purushottam Padmanabhan and Shaik Iqbal Ahmed and Avinash Bardia and Aleem Ahmed Khan},

url = {https://jmas.in/index.php?mno=49688},

doi = {10.5455/jmas.49688},

issn = {2231-1696},

year  = {2021},

date = {2021-07-31},

urldate = {2021-01-01},

journal = {Journal of Medical and Allied Sciences},

volume = {11},

number = {2},

pages = {105-113},

publisher = {Deccan College of Medical Sciences},

abstract = {It has been estimated that 4.4 billion individuals are infected with Helicobacter pylori and the prevalence of the infection varies globally ranging from the highest reported in Africa (70.1%) to lowest in Switzerland (18.9%). The prevalence is still abundant in developing countries and is due to the outcome of low socioeconomic status and poor sanitation. Helicobacter pylori infection is implicated in several pathological states and is confronting a gigantic challenge to the global health community. This review provides an extensive picture of Helicobacter pylori infection prevalence and its association with disease outcomes using myriad sample studies, emphasizing detection using gastric biopsy. Moreover novel molecular mechanisms unveiled to date are also presented to better comprehend the underlying events that triggers the epidemiological effects. Data and mechanistic pathways presented in this review aid in improved regulation of Helicobacter pylori infection and assists in designing and crafting new drugs to treat the associated clinical conditions.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Butul_2021,

title = {Onychomadesis, a post infectious sequelae of Hand-Foot-and-Mouth disease: A case report},

author = {Bushra Butul and Neda Fatima and Sumayya Fathima and Mohammed Nazim Uddin},

url = {https://jmas.in/index.php?mno=61243},

doi = {10.5455/jmas.61243},

issn = {2231-1696},

year  = {2021},

date = {2021-07-31},

urldate = {2021-01-01},

journal = {Journal of Medical and Allied Sciences},

volume = {11},

number = {2},

pages = {183-185},

publisher = {Deccan College of Medical Sciences},

abstract = {Onychomadesis is an idiopathic shedding of a proximal part of the nail as a result of the temporary suspension of a nail matrix function. It is seen in fingernails, toenails, or both. Various underlying causes like systemic illness, drug reactions, Viral diseases, and bullous diseases have been associated with Onychomadesis. However, the mechanism of any such association remains unclear. Here we present a case of Onychomadesis and Beau lines in the fingernails of a 13-month-old girl following Hand, foot, and mouth disease.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Prasad_2021c,

title = {Status of vitamin D receptor gene polymorphism and 25-hydroxy vitamin D deficiency with essential hypertension},

author = {M. Prasad and D. Rajarajeswari and P. Aruna and K. Ramalingam and R. Viswakumar and Nusrath Fathima and Sandeep Kumar Vishwakarma and Aleem Ahmed Khan},

url = {https://link.springer.com/content/pdf/10.1007/s12291-021-00984-z.pdf?pdf=button},

doi = {10.1007/s12291-021-00984-z},

issn = {0974-0422},

year  = {2021},

date = {2021-06-15},

urldate = {2021-06-01},

journal = {Indian Journal of Clinical Biochemistry},

volume = {37},

issue = {3},

pages = {335-341},

publisher = {Springer Science and Business Media LLC},

abstract = {Essential hypertension (EH) is a multifactorial and complex disease with high rate of incidence and associated co-morbidities. Previous studies do not provide unanimous results for the risk of hypertension and association with Fok I genotype frequency and serum vitamin D levels. Hence, this study was undertaken to determine the status of Fok I vitamin D receptor (VDR) gene polymorphism along with vitamin D levels and blood pressure in patients with EH. Four hundred (200 controls and 200 cases of essential hypertension) participants from general Indian population were enrolled in this study. Peripheral blood samples were collected for genotyping Fok I-VDR gene polymorphism using PCR–RFLP method whereas 25-OH vitamin D levels in serum were quantified using high performance liquid chromatography (HPLC). Significantly reduced 25-OH vitamin D levels were observed in patients with EH (24.04 ± 8.62 vs 50.46 ± 15.46) compared to control subjects (p = 0.0001). Homozygous recessive genotype ‘ff’ frequency was increased by 8.06 fold (CI: 3.71–17.47},

key = {pmid34149207},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tiwari_2021,

title = {Efficacy and safety of neural stem cell therapy for spinal cord injury: a systematic literature review},

author = {Santosh Tiwari and Shaswati Khan and Shiva V. Kumar and Rohit Rajak and Asma Sultana and Shaik Abjal Pasha and Divya Gauba and Pinaki Ghosh and Tanu Khurana and Aishwarya Kulkarni and Yugandhar P. Reddy and Aleem Ahmed Khan and Varun Kumar Sharma},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0040595720301165?via%3Dihub},

doi = {10.1016/j.therap.2020.06.011},

issn = {0040-5957},

year  = {2021},

date = {2021-05-29},

urldate = {2021-05-01},

journal = {Therapies},

volume = {76},

issue = {3},

pages = {201-210},

publisher = {Elsevier BV},

abstract = {Aims: To summarize the evidence on the efficacy and safety of neural stem cell therapy (NSCT) for the treatment of spinal cord injury (SCI). Methods: A systematic literature review of Medline®, EMBASE® and Cochrane library was performed to identify studies reporting efficacy and safety of NSCT in SCI. Articles were included if they reported efficacy and safety data of SCI patients who received NSCT. Results: Overall, four studies of the 277 records met all the study eligibility criteria. Over the 1-year follow-up period, motor scores were significantly higher among patients who received NSCT compared with those who did not (American Spinal Injury Association [ASIA] motor scores (mean ± standard deviation [SD]): 7.9 ± 1.2 versus 3.9 ± 0.6; upper extremity motor score: 7.8 ± 2.1 versus 3.9 ± 0.6, both P < 0.05). Sensory scores (pinprick score: 4.8 ± 1.3 versus 2.9 ± 0.6; P = 0.5; light touch score: 6.9 ± 3.1 versus 2.3 ± 0.5, P = 0.3), ASIA impairment scale (26% versus 7%) or pain score (baseline: 2.4 ± 0.6; 1-year: 3.4 ± 0.4) were comparable in both NSCT and non-NSCT cohorts. Over the 1-year follow-up period, the graded redefined assessment of strength, sensibility, and prehension and international standards for neurological classification of SCI scores showed a mean improvement of 14.8 and 17.8 points respectively. Overall, treatment with NSCT showed favorable safety and tolerability profile. Conclusions: Due to the limited and poor-quality evidence, it is too early to make robust conclusions on the efficacy of NSCT in the treatment of SCI. However, based on the included studies, NSCT seems to be a potential option worth exploring among patients with SCI. Nonetheless, prospective, randomized trials in larger cohorts are needed to validate the efficacy and safety of NSCT in the treatment of SCI.},

key = {pmid32709426},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sharma_2021,

title = {COVID-19 and anticoagulant based therapeutics: approach with great promise},

author = {Varun Kumar Sharma and Lomas Kumar Tomar and Charu Tyagi and Jayanad Manjhi and Yugandhar P. Reddy and Santosh Kumar Tiwari},

url = {https://pdf.sciencedirectassets.com/277405/1-s2.0-S1876034121X00056/1-s2.0-S1876034120306705/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEJ7%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJIMEYCIQDRAeWS4w8DsEvBaCWdCCuNM1YicXv4J3wTC1LEosts%2FAIhAKijTjbvtywEIEJAyq2lx%2B%2BuZZCypK2jZGkus7%2F2Jh%2BwKrwFCLf%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEQBRoMMDU5MDAzNTQ2ODY1Igx3S2shtPadWqHfXQsqkAXcqnaQVDjdCUdsvEPOzY4szEHZ9Os1n8UfHkx67W%2BYSQ28vC1Tj50c0fxvDhkhNx8ddWLPqt1C%2BcVWpiH4uiN3lnyDIRpg19fREcvxuO%2F1VCn2k%2FKId%2B5zZ9fo9%2Bq3wFt%2FDNqv%2BaQobmqvtZkbS5EsvVNHsCHNjw1HvqjjWzZQ5g%2Bdg%2FdZom1%2BwDe%2B1sxa8m9H7APJQ04MKe5PKtw7qMGn5f57VBsfHBJAs7zDfjxFQC%2BgwEG%2Bl3kv04UTzxdw3%2BgnvOAz5I7teS8BJFYmGOvWaOnsopO1qTdtazOJQT1GUEdHD7p02o0XyyLkvw%2BmUniGPF3ICRgRyUeMyx2phY335gRv4MmUSV6JqpN1%2FeWSz0h75XmyZRAOXl7ErokQPbvrFWem7gQfIGdRqRQ0BKj0twbZNZsWtllff9EuVgMjAXePNkYOM004G6hDPK6%2BNDBq2Dn0JUo%2BG1JmDsOKqzHO6Myu0ulHpiw51qfxKndLQan0IOqUHS%2FuX2ZcatXnv5j9835PxoJp5OiZACXFsma1sisGKBmtjzURvY7F2ik0rvwWGdk5O2eS9mVap7LQEezUm388xg1deZqIElr7FV6Les7tkekow38O5VeaPiElVH%2FF2YS7G%2BmDFakx8vWH1GmB2fWbtbRJMTs9pf0h2jLA9iZ6C%2FxaQoX9t0BUu0PWeWm09E6YzMo8dh47nvZL70oB7VAOXVc%2FN2F9RRap8xEnCkJ1Xso2z9X1p4%2BQvEoi2SDWNQ%2F91jKxDfRf3i62TJ2OfD1OFx1zjaFpAe71FyL%2F9vY1NNRJ%2FsF%2FFhKsLG7kDm9go%2BRow%2BYt1H3PSXkQwg9oI7k%2B%2FxyJYgd4R3y9HsQLi2YIq22LKsfyC%2BvbDVqh2jCbg8OpBjqwAYooU%2B1d%2F%2FmgCu90yJMNVnlEUzrQcQORrCAO8RdhUBI5%2BvI8ac2aMlfCxJf5j5SBQb8GVj1cuEi5q5YugmPU3yDul3wd6wHmF%2F7qbH6BOFbVnE7hWVUoQ59dRZNoMfQmsis25%2Fa%2BxnYh%2BKax5I7QDuEbr9cbLT0WtZ%2FgWM80GDHRV8Qhg%2F2X1EG58I1AvJFS88cBfj%2Bg6o1ofoEiSlzjwtodnWuxH6E0J8gTt8po4gZE&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20231019T063617Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTYT464ESVU%2F20231019%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=26a752bdcff19ceb155bb926d691b3fdc40740e4fe38f6b63a11379fd74acaac&hash=c871e24d084704d2c1780633b4aa6abc480224f0a003e7953687ccf6ddab6f92&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S1876034120306705&tid=spdf-ce493a3c-fcf1-423e-818f-18a542be280a&sid=5010876e6fc130474c4b47e5adff7fcf6bb4gxrqb&type=client&tsoh=d3d3LnNjaWVuY2VkaXJlY3QuY29t&ua=0f0d57515b5054555650&rr=818701809fef2e9f&cc=in},

doi = {10.1016/j.jiph.2020.09.014},

issn = {1876-0341},

year  = {2021},

date = {2021-05-01},

urldate = {2021-05-01},

journal = {Journal of Infection and Public Health},

volume = {14},

issue = {5},

pages = {681-682},

publisher = {Elsevier BV},

key = {pmid33248908},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2020,

title = {Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration},

author = {Sandeep Kumar Vishwakarma and Shahana Sarwar and Mohammed Abdul Majid Adil and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/abs/pii/S004081662030433X?via%3Dihub},

doi = {10.1016/j.tice.2020.101443},

issn = {0040-8166},

year  = {2020},

date = {2020-12-01},

urldate = {2020-12-01},

journal = {Tissue and Cell},

volume = {67},

pages = {101443},

publisher = {Elsevier BV},

abstract = {Introduction: Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment. Current therapeutic approaches including enterocystoplasty have several limitations. Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study. Materials and methods: An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter. The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis. Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration. Results: The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min. The DUB scaffold retains organ architecture, ECM composition, and mechanical strength. No significant amount of residual nucleic acid was observed post-decellularization. Furthermore, MSCs derived from human UCB engrafted and proliferated well on DUB scaffolds in highly aligned manner under xeno-free condition. Conclusion: Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.},

key = {pmid32998076},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Afreen_2020b,

title = {Single nucleotide polymorphism in the promoter region of interleukin-4 and risk of asthma: TT homozygotes may have high propensity of developing chronic airway remodeling},

author = {Arshiya Afreen and S. Farhana F. A. and Sumaya Fatima and T. K. M. Rayees and Nida Makeen and Nusrath Fathima and Ashfaq Hasan and M. Aleemuddin Naveed and Fahad Abdullah Bin Breik and Mohd. Mohiuddin and Mohammed Ishaq},

url = {https://www.ijrrjournal.com/IJRR_Vol.7_Issue.9_Sep2020/IJRR0014.pdf},

issn = {2349-9788},

year  = {2020},

date = {2020-09-01},

urldate = {2020-09-01},

journal = {International Journal of Research and Review},

volume = {7},

issue = {9},

pages = {104-110},

abstract = {Background: In view of a key role played by Interleukin-4 (IL-4) in the molecular mechanisms leading to the development of asthma and its complications like chronic airway remodeling, a few reports have attempted to investigate a possible role of SNP’s in the promoter region to predict genetic risk. As it is associated with quantitative variations in IL-4 gene expression and may serve as an important candidate gene for asthma risk prediction. Objective: To investigate the polymorphic variations, at -590 (C/T) in the promoter region of IL-4 gene. Methods: 50 consecutively selected cases of asthma (36 females and 14 males) were recruited and genotyping for SNP at -590 C/T was done by PCR-RFLP method on the genomic DNA extracted from blood samples. Blood samples from 50 randomly selected healthy subjects served as controls. Mean values of Absolute Eosinophil Count and total serum IgE between the genotypes were compared by student’s unpaired t test. Odds-ratio analysis was carried out to determine risk of developing asthma. Results: Odds ratio (OR) analysis revealed two-and-a-half folds increased risk of asthma for individuals with TT genotype compared to those with CC genotype. A decreased frequency of CC genotype in patients (28%) compared to controls (58%) is indicative of protective nature of CC genotype. AEC counts and total serum IgE levels were significantly higher in TT compared to CC and CT genotypes. Conclusion: It is concluded that individuals with TT and CT genotypes (overproducers of IL-4) have high risk of developing asthma and high vulnerability to develop chronic airway remodeling.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Reddy_2020,

title = {Fluoride-induced expression of neuroinflammatory markers and neurophysiological regulation in the brain of wistar rat model},

author = {Yugandhar P. Reddy and Santosh Tiwari and Lomas K. Tomar and Nalini Desai and Varun Kumar Sharma},

url = {https://link.springer.com/article/10.1007/s12011-020-02362-x},

doi = {10.1007/s12011-020-02362-x},

issn = {0163-4984},

year  = {2020},

date = {2020-08-31},

urldate = {2020-08-01},

journal = {Biological Trace Element Research},

volume = {199},

issue = {7},

pages = {2621-2626},

publisher = {Springer Science and Business Media LLC},

abstract = {Excess fluoride intake has been linked with various pathological conditions. The objective of the present study was to understand the role of fluoride in neurotoxic, neuroinflammatory, and neurodegenerative changes in the brain tissue of Wistar rats. Wistar rats were fed with water containing 20–100 ppm (ppm) sodium fluoride (NaF). An array of neurotransmitters (acetylcholine, dopamine, epinephrine, norepinephrine, serotonin, histamine, and glutamate) expression levels were estimated with respect to different fluoride concentrations. Additionally, its effect on the expression levels of specific neuroinflammatory markers (iNOS, COX-2, TNF-α, PKC, VEGF, and HSP-70) in brain tissues of Wister rats was assessed. An increase in NaF concentration resulted in increased fluoride deposition in the brain which in turn caused increase levels of epinephrine, histamine, serotonin, and glutamate and decreased levels of norepinephrine, acetylcholine, and dopamine in a dose-dependent manner. Tissue fluoride levels of the hippocampus, neocortex, cerebellum, spinal cord, and sciatic nerve increased significantly in fluoride fed rats. Transmission electron microscopy in the experimental animals revealed axon deterioration, myelin sheath degeneration, and dark cells with scanty cytoplasm in the spinal cord and sciatic nerve. Additionally, vacuolated swollen mitochondria were observed in the neocortex, hippocampus, and cerebellum. Results suggest excess fluoride intake modulates a set of biological marker and promote neuroinflammatory and neurodegenerative condition in Wister rats. Therefore, we conclude that the accumulation of NaF alters the neurological function which leads to neurodegenerative disorders.},

key = {pmid32865723},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jameel_2019,

title = {Prevalence of auto-immune thyroid disease and anti-TPO antibodies in patients visiting out-patient ward for routine health check-up},

author = {Tahmeen Jameel and Mizba Khanam and Qursheed Sultana and Mohammed Ishaq},

year  = {2019},

date = {2019-10-01},

urldate = {2019-10-01},

journal = {IOSR Journal of Biotechnology and Biochemistry},

volume = {5},

issue = {5},

pages = {39-41},

abstract = {The objective of the present study was to investigate prevalence of Auto-Immune Thyroid Disease (AITD) and screening for anti-thyroid peroxide auto antibodies (anti-TPO) in subjects visiting out-patient ward of Department of Medicine (Princess Esra Hospital, Hyderabad). A total of 136 cases were included consecutively. Age and gender of the patients were recorded and serum was tested for Thyroid Profile and anti-TPO antibodies by Enzyme Linked ImmunoSorbent Assay (ELISA). The male to female ratio in 136 cases was 6.4%:93.6%. It was observed that 36 cases (26%) were found to be suffering from thyroid disease based on TSH, T3 and T4 levels of these 16 (11.7%) were hypothyroid, 7 (5%) were hyperthyroid cases. The remaining 10 (7%) cases were identified as having subclinical hypothyroidism. Of these cases 34 were found to be positive for anti-TPO auto-antibodies. The 2 negative cases were of hypothyroidism. Inordinately high proportion of females were found among AITD cases as well as among all the 136 cases selected for the study.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tiwari_2019,

title = {Gene expression patterns of COX-1, COX-2 and iNOS in H. pylori infected histopathological conditions},

author = {Santosh K. Tiwari and Asma Sultana Shaik and Abjal Pasha Shaik and Abdullah A. Alyousef and Avinash Bardia and Md. Aejaz Habeeb and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0882401019308009?via%3Dihub},

doi = {10.1016/j.micpath.2019.103634},

issn = {0882-4010},

year  = {2019},

date = {2019-10-01},

urldate = {2019-10-01},

journal = {Microbial Pathogenesis},

volume = {135},

pages = {103634},

publisher = {Elsevier BV},

abstract = {Background: Research indicates that Helicobacter pylori can inflict severe histological damage through the modulation of host-related genes. The current study investigated the effect of H. pylori genotypes in the outcome of disease, and the expression of anti-apoptotic related genes, COX-1, COX-2, and iNOS genes in benign, pre-malignant, and malignant lesions of gastric carcinogenesis. Materials and methods: Tissue samples from H. pylori positive patients were graded based on the genotype of the infected H. pylori strain. Expression of COX-1, COX-2 and iNOS was assessed using a combination of real-time PCR and immunohistochemistry. Results: Gene expression studies confirmed that COX-2 and iNOS expression was highly and selectively induced in epithelium with premalignant changes such as atrophic conditions, metaplasia and dysplasia, suggesting an important role of these genes in the sequence to gastric carcinoma of the intestinal type. Furthermore, the expression of COX-2 and iNOS was also dependent on the genotype of H. pylori and subjects with genotype-1 exhibited significantly higher expressions of COX-2 and iNOS compared to other genotypes. Comparison of the expression levels among infected and uninfected individuals demonstrated significant difference in the expression pattern of COX-2 gene whereas iNOS expression was found only in subjects infected H. pylori (p < 0.001). Immunohistochemical staining showed 1.5619 folds higher propensity of COX-2 and 3.2941 folds higher intensity of iNOS expression in subjects infected with H. pylori genotype 1. Conclusion: The up-regulation of COX-2 and iNOS was associated with the genotype of the H. pylori strain and the presence of certain genotype may greatly affect early events during carcinogenesis.},

key = {pmid31325568},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2019,

title = {Intraperitoneal transplantation of bioengineered humanized liver grafts supports failing liver in acute condition},

author = {Sandeep Kumar Vishwakarma and Avinash Bardia and Chandrakala Lakkireddy and Nagarapu Raju and Syed Ameer Basha Paspala and Md. Aejaz Habeeb and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/pii/S0928493117344946?via%3Dihub},

doi = {10.1016/j.msec.2019.01.045},

issn = {0928-4931},

year  = {2019},

date = {2019-05-01},

urldate = {2019-05-01},

journal = {Materials Science and Engineering: C},

volume = {98},

pages = {861-873},

publisher = {Elsevier BV},

abstract = {Acute liver failure (ALF) is one of the most devastating fatal conditions which have posed crucial challenges to the clinicians and researchers for identifying permanent cure. Currently liver transplantation has been considered as the only managerial option. However it's wider applicability has been limited owing to non-availability of quality donor organs, cost-intensiveness, surgical hitches, life-long use of immunosuppressive drugs and long-term complications. Since last decades, several liver support systems have been developed for the management of failing liver in acute condition. However, the major limitation has been the lack of natural biological support and long-term survival of the grafts post-transplantation. Repopulation of decellularized xenogeneic organs is one of the emerging technologies for development of humanized neo-organs for demanding regenerative application. However, the earlier reported studies do not fulfil the insistence to provide immunologically tolerable humanized liver grafts for clinical applications. Here we demonstrate an efficient approach to generate transplantable humanized liver grafts which provides long-term support to the failing liver in Acute Liver Failure (ALF) animal models. These bioengineered humanized liver tissue grafts expresses several liver specific transcripts and performed crucial synthetic (albumin production) and detoxification (urea synthesis) functions at comparative level to normal liver. Intraperitoneal transplantation of these humanized liver grafts offered favourable microenvironment to exchange toxic substances across the barrier during ALF condition and provided long-term survival and function of the graft. In summary, the results of present study provide a first proof of concept in pre-clinical ALF animal model for the applicability of these bioengineered humanized livers in the management of failing liver on demand and may be considered as potential bridge to liver transplantation.},

key = {pmid30813092},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2019b,

title = {Engineering bio-mimetic humanized neurological constructs using acellularized scaffolds of cryopreserved meningeal tissues},

author = {Sandeep Kumar Vishwakarma and Chandrakala Lakkireddy and Avinash Bardia and Syed Ameer Basha Paspala and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/pii/S0928493118339328?via%3Dihub},

doi = {10.1016/j.msec.2019.04.028},

issn = {0928-4931},

year  = {2019},

date = {2019-04-12},

urldate = {2019-09-01},

journal = {Materials Science and Engineering: C},

volume = {102},

pages = {34-44},

publisher = {Elsevier BV},

abstract = {Spinal cord injury (SCI) is one of the most precarious conditions which have been one of the major reasons for continuous increasing mortality rate of SCI patients. Currently, there is no effective treatment modality for SCI patients posing major threat to the scientific and medical community. The available strategies don't mimic with the natural processes of nervous tissues repair/regeneration and majority of the approaches may induce the additional fibrotic or immunological response at the injury site and are not readily available on demand. To overcome these hurdles, we have developed a ready to use bioengineered human functional neurological construct (BHNC) for regenerative applications in SCI defects. We used cryopreserved meningeal tissues (CMT) for bioengineering these neurological constructs using acellularization and repopulation technology. The technology adopted herein generates intact neurological scaffolds from CMT and retains several crucial structural, biochemical and mechanical cues to enhance the regenerative mechanisms. The neurogenic differentiation on CMT scaffolds was almost similar to the freshly prepared meningeal scaffolds and mimics with the natural nervous tissue developmental mechanisms which offer intact 3D-microarchitecture and hospitable microenvironment enriched with several crucial neurotrophins for long-term cell survival and function. Functional assessment of developed BHNC showed highly increased positive staining for pre-synaptic granules of Synapsis-1 along with MAP-2 antibody with punctuate distribution in axonal regions of the neuronal cells which was well supported by the gene expression analysis of functional transcripts. Given the significant improvement in the field may enable to generate more such ready to use functional BHNC for wider applicability in SCI repair/regeneration.},

key = {pmid31147006},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fathima_2019,

title = {Association and gene–gene interaction analyses for polymorphic variants in CTLA-4 and FOXP3 genes: role in susceptibility to autoimmune thyroid disease},

author = {Nusrath Fathima and Parimala Narne and Mohammed Ishaq},

url = {https://link.springer.com/article/10.1007/s12020-019-01859-3},

doi = {10.1007/s12020-019-01859-3},

issn = {1559-0100},

year  = {2019},

date = {2019-02-15},

urldate = {2019-02-01},

journal = {Endocrine},

volume = {64},

issue = {3},

pages = {591-604},

publisher = {Springer Science and Business Media LLC},

abstract = {Purpose: Polymorphic variants of cytotoxic T-lymphocyte antigen-4 (CTLA-4) and forkhead box protein P3 (FOXP3) genes are implicated in dysregulated immune homeostasis and autoimmune disorders. We analyzed the association between CTLA-4 rs231775 and FOXP3 rs3761548, rs3761549 polymorphisms and predisposition to autoimmune thyroid disease (AITD), inclusive of Hashimoto’s thyroiditis (HT) and Graves’ disease (GD) in South-Indian population. Methods: A total of 355 AITD subjects (comprising 275 HT and 80 GD) and 285 randomly selected age- and sex-matched control subjects were genotyped for the aforementioned polymorphisms by PCR-RFLP method. Results: The rs231775 “G” allele was preponderant in HT and GD subjects when compared with controls and exerted a dominant influence on the susceptibility to HT (p = 0.009) and GD (p = 0.02), respectively. There was no allelic association of rs3761548 and rs3761549 polymorphisms with AITD susceptibility, albeit a significant difference in genotype distribution with respect to rs3761549. Haplotype analysis revealed an increased frequency of rs3761548 “C”–rs3761549 “T” in HT and GD subjects, thereby associating it with disease predisposition (p = 0.03). Epistatic interaction analysis by multifactor dimensionality reduction approach revealed redundancy between CTLA-4 and FOXP3 genes in influencing the susceptibility to AITD. Conclusions: The genetic variation in CTLA-4 gene with reference to rs231775 polymorphism contributes to an increased predisposition to HT and GD. Also, in conjunction with FOXP3 gene variants it seems to influence the susceptibility to HT and GD respectively. The significance of these findings in combination with antithyroid antibody screening could plausibly contribute towards meticulous case-finding for effective treatment of HT and GD.},

key = {pmid30771152},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Naz_2019,

title = {Activation of integrated stress response pathway regulates IL-1β production through posttranscriptional and translational reprogramming in macrophages},

author = {Saima Naz and Srikanth Battu and Rafiq Ahmad Khan and Sumbul Afroz and Jeevan Giddaluru and Sandeep Kumar Vishwakarma and Vishnupriya Satti and Md. Aejaz Habeeb and Aleem Ahmed Khan and Nooruddin Khan},

url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.201847513},

doi = {10.1002/eji.201847513},

issn = {0014-2980},

year  = {2019},

date = {2019-01-01},

urldate = {2019-01-01},

journal = {European Journal of Immunology},

volume = {49},

issue = {2},

pages = {277-289},

publisher = {Wiley},

abstract = {Immune cells sense and programme its cellular machinery appropriately to the environmental changes through the activation of cytoprotective adaptive pathway so-called the “integrated stress response (ISR)”. However, the mechanisms implicated in ISR-induced protective responses are poorly understood. Here, we show that ISR activation by arsenite (Ar) results in suppression of IL-1β production in macrophages and inhibition of DSS-induced colitis in a murine model through a novel posttranscriptional and translation regulatory (PTR) mechanism. Ar triggers PTR events through eIF2α-phosphorylation, which results in the attenuation of active polysome formation leading to the accumulation of translationally stalled IL-1β mRNAs. Translationally stalled IL-1β mRNAs recruit RNA-binding proteins (TIA-1/TIAR), resulting in the formation of RBP-RNA complexes known as stress granules (SGs). The SGs bound IL-1β mRNAs might undergo degradation through induction of autophagy. Also, we show that Ar posttranslationally impairs processing and secretion of IL-1β by diminishing inflammasome activation. Altogether, this study unveils a novel mechanism of IL-1β regulation and further suggests that pharmacological activation of cytoprotective ISR pathway might provide an effective therapeutic intervention against inflammatory diseases.},

key = {pmid30578631},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vanka_2019,

title = {Osteo/odontogenic differentiation of human mesenchymal stem cells with platelet-rich plasma and mineral trioxide aggregate},

author = {Shanthi Vanka and Amit Vanka and Sandeep Kumar Vishwakarma and Manohar K. Bhat and Othman Wali and Aleem Ahmed Khan},

url = {https://www.thejcdp.com/doi/pdf/10.5005/jp-journals-10024-2677},

doi = {10.5005/jp-journals-10024-2677},

issn = {1526-3711},

year  = {2019},

date = {2019-01-01},

urldate = {2019-01-01},

journal = {The Journal of Contemporary Dental Practice},

volume = {20},

issue = {10},

pages = {1171-1178},

publisher = {Jaypee Brothers Medical Publishing},

abstract = {Aim: Aim of the study was to investigate the effect of PRP and MTA individually and combined on in vitro human bone marrow mesenchymal stem cells’ (MSCs) proliferation and osteo/odontogenic differentiation potential. Materials and methods: MSCs were cultured in vitro with MTA, 5% PRP, 10% PRP, MTA with 5%PRP, and MTA with 10% PRP. Fetal calf serum (FCS) was used as control. Cell viability and proliferative efficiency were tested with cell adhesion and MTT assay. Osteo/odontogenic differentiation was assessed and quantified with alizarin red staining. Results: MTA alone, MTA with 5% PRP, and MTA with 10% PRP showed significantly high proliferation at day 7 and 14 when compared to the control group. Enhanced differentiation and the highest calcium deposition was observed in MTA with the 10% PRP group. Conclusion: Within limitations of the in vitro environment, results imply an increased proliferation and induction of MSCs into osteo/odontogenic differentiation by the combination rather than a mere sealing of PRP by MTA. Clinical significance: PRP and MTA have the potential for true regeneration of the pulp tissue. Moreover, the combination of PRP and MTA can 

be utilized to expand the MSCs to generate adequate numbers for clinical applications, without xenogenic contamination.},

key = {pmid31883252},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2018b,

title = {Bioengineered functional humanized livers: an emerging supportive modality to bridge the gap of organ transplantation for management of end-stage liver diseases},

author = {Sandeep Kumar Vishwakarma and Chandrakala Lakkireddy and Avinash Bardia and Syed Ameer Basha Paspala and Chaturvedula Tripura and Md. Aejaz Habeeb and Aleem Ahmed Khan},

url = {https://f6publishing.blob.core.windows.net/fbbe1f7d-af5e-44ac-9cbd-ad7683f01680/WJH-10-822.pdf},

doi = {10.4254/wjh.v10.i11.822},

issn = {1948-5182},

year  = {2018},

date = {2018-11-27},

urldate = {2018-11-27},

journal = {World Journal of Hepatology},

volume = {10},

issue = {11},

pages = {822-836},

publisher = {Baishideng Publishing Group Inc.},

abstract = {End stage liver diseases (ESLD) represent a major, neglected global public health crisis which requires an urgent action towards finding a proper cure. Orthotropic liver transplantation has been the only definitive treatment modality for ESLD. However, shortage of donor organs, timely unavailability, post-surgery related complications and financial burden on the patients limits the number of patients receiving the transplants. Since last two decades cell-based therapies have revolutionized the field of organ/tissue regeneration. However providing an alternative organ source to address the donor liver shortage still poses potential challenges. The developments made in this direction provide useful futuristic approaches, which could be translated into pre-clinical and clinical settings targeting appropriate applications in specific disease conditions. Earlier studies have demonstrated the applicability of this particular approach to generate functional organ in rodent system by connecting them with portal and hepatic circulatory networks. However, such strategy requires very high level of surgical expertise and also poses the technical and financial questions towards its future applicability. Hence, alternative sites for generating secondary organs are being tested in several types of disease conditions. Among different sites, omentum has been proved to be more appropriate site for implanting several kinds of functional tissue constructs without eliciting much immunological response. Hence, omentum may be considered as better site for transplanting humanized bioengineered ex vivo generated livers, thereby creating a secondary organ at intra-omental site. However, the expertise for generating such bioengineered organs are limited and only very few centres are involved for investigating the potential use of such implants in clinical practice due to gap between the clinical transplant surgeons and basic scientists working on the concept evolution. Herein we discuss the recent advances and challenges to create functional secondary organs through intra-omental transplantation of ex vivo generated bioengineered humanized livers and their further application in the management of ESLD as a supportive bridge for organ transplantation.},

key = {pmid30533183},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2018c,

title = {Bioengineering human neurological constructs using decellularized meningeal scaffolds for application in spinal cord injury},

author = {Sandeep Kumar Vishwakarma and Avinash Bardia and Chandrakala Lakkireddy and Syed Ameer Basha Paspala and Aleem Ahmed Khan},

url = {https://www.frontiersin.org/articles/10.3389/fbioe.2018.00150/full},

doi = {10.3389/fbioe.2018.00150},

issn = {2296-4185},

year  = {2018},

date = {2018-11-01},

urldate = {2018-11-01},

journal = {Frontiers in Bioengineering and Biotechnology},

volume = {6},

publisher = {Frontiers Media SA},

abstract = {Spinal cord injury (SCI) is one of the most devastating conditions echoes with inflammation, enhanced fibrosis and larger axonal gaps due to destruction of neurological cells which has caused continuous increasing mortality rate of SCI patients due to absence of suitable treatment modalities. The restoration of structural and functional aspect of damaged neurological tissues at the lesion site in spinal cord has been challenging. Recent developments have showed tremendous potential of neural stem cell-based strategies to form a neuronal relay circuit across the injury gap which facilitates some levels of improvement in SCI condition. However, to provide better therapeutic responses, critical mass of grafted cells must survive for long-term and differentiate into neuronal cells with well-developed axonal networks. Hence, development of tissue specific biological neuronal constructs is highly desirable to provide mechanical and biological support for long-term survival and function of neurological cells within natural biological niche. In this study, we report development of a tissue specific neuronal constructs by culturing human neural precursor cells on decellularized meningeal scaffolds to provide suitable biological neuronal construct which can be used to support mechanical, structural and functional aspect of damaged spinal cord tissues. This particular tissue specific biological construct is immunologically tolerable and provides precisely orchestral three-dimensional platform to choreograph the long-distance axonal guidance and more organized neuronal cell growth. It passes sufficient mechanical and biological properties enriched with several crucial neurotrophins required for long-term survival and function of neurological cells which is required to form proper axonal bridge to regenerate the damaged axonal connectomes at lesion-site in SCI.},

key = {pmid30443545},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2018,

title = {Molecular dynamics of pancreatic transcription factors in bioengineered humanized insulin producing neoorgan},

author = {Sandeep Kumar Vishwakarma and Chandrakala Lakkireddy and Avinash Bardia and Nagarapu Raju and Syed Ameer Basha Paspala and Md. Aejaz Habeeb and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0378111918307753?via%3Dihub},

doi = {10.1016/j.gene.2018.07.006},

issn = {0378-1119},

year  = {2018},

date = {2018-10-30},

urldate = {2018-10-30},

journal = {Gene},

volume = {675},

pages = {165-175},

publisher = {Elsevier BV},

abstract = {Background: The present study has been aimed to identify molecular dynamics of pancreatic transcription factors (pTFs) during events of directed trans-differentiation of human hepatic progenitor cells (hHPCs) into insulin producing cells (InPCs) within bioengineered humanized neoorgan. The study demonstrates applicability of acellularized whole splenic scaffold (ASOS) to generate insulin producing humanized transplantable neoorgan through activation of pancreatic transcription factors. Methods: An efficient acellularization process was developed for xenogeneic rat spleen using change in different gradients of reagents perfusion through splenic artery for varying time points. The acellularized xenogeneic spleen scaffold was characterized thoroughly for preservation of extra-cellular matrix and retention of organ specific vasculature and mechanical properties. Further scaffolds were sterilized and repopulated with hHPCs which were triggered using a stage wise induction with growth factors and hyperglycemic challenge for trans-differentiation into InPCs. Dynamics of pTFs alone or simultaneously during induction process was identified using gene expression analysis and immunological staining. Results: The cells within the engineered neoorgan respond to growth factors and extrinsic hyperglycemic challenge and generate large number of InPCs under controlled dynamic regulation of pTFs. Highly controlled regulation of pTFs generates higher percentage of Nkx-6.1+/C-peptide+ cells within the engineered splenic scaffolds. Generation of high percentage of insulin and C-peptide positive cells in three-dimensional organ architecture responded better to hyperglycemic stimuli and produced higher quantity of insulin than 2D-culture system. Conclusion: The present study provides a novel platform for designing effective regenerative strategies using whole organ scaffolds to control hyperglycemia under tight regulation of pTFs using humanized neoorgan system.},

key = {pmid30180963},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Naz_2018,

title = {Transcriptome meta-analysis identifies immune signature comprising of RNA binding proteins in ulcerative colitis patients},

author = {Saima Naz and Rafiq Ahmad Khan and Jeevan Giddaluru and Srikanth Battu and Sandeep Kumar Vishwakarma and Mabu Subahan and Vishnupriya Satti and Nooruddin Khan and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0008874918302600?via%3Dihub},

doi = {10.1016/j.cellimm.2018.09.003},

issn = {0008-8749},

year  = {2018},

date = {2018-09-21},

urldate = {2018-12-01},

journal = {Cellular Immunology},

volume = {334},

pages = {42-48},

publisher = {Elsevier BV},

abstract = {Ulcerative colitis (UC) is a persistent inflammatory illness, which is clinically categorised as Inflammatory bowel disease (IBD), affecting millions of people worldwide. The precise cause behind the pathology of the disease remains unknown. However, the involvement of multiple factors including genetic predisposition, immunological deregulations, microbiota imbalance, and environmental triggers has been suggested. Amongst all these factors, the over-active immunological response reported in UC patients seems to be a promising target for therapy. Moreover, identification of gene signatures associated with disease onset and progression would help in better understanding of the molecular mechanisms involved in the disease pathogenesis. Here, we have conducted meta-analysis of gene expression profiles of UC patient microarray datasets accessible in public databases and further validated the in-silico findings in UC patients’ blood samples. Our study reveals that UC pathogenesis perturbs expression of several inflammatory genes. In addition, we report a novel gene signature comprising of TIA1 (T cell restricted intracellular antigen) and TIAR (TIA1 related protein; also known as TIAL1), which were found to be significantly downregulated in UC patients. TIA1 and TIAR are RNA-binding proteins (RBPs), which function as a translational represser by binding to ARE sequences in the 3′ UTR of mRNAs encoding inflammatory mediators including cytokines. Our findings demonstrate that deletion of TIAR using gene specific siRNAs in-vitro results in enhanced production of inflammatory cytokine IL-1β. In conclusion, the findings of this study reveal that down regulation of TIA1/TIAR genes could be responsible for UC associated inflammation. This study highlights the usefulness of the meta-analysis approach in the identification of unique gene signatures that might deliver mechanistic insights into UC pathogenesis and possibly assist in discovery of prognostic markers and therapeutic interventions.},

key = {pmid30327138},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2018d,

title = {Association of CD14 and macrophage migration inhibitory factor gene polymorphisms with inflammatory microRNAs expression levels in ankylosing spondylitis and polyarthralgia},

author = {Sandeep Kumar Vishwakarma and Chandrakala Lakki Reddy and G. Sravani and B. V. S. Sastry and N. Raju and S. I. Ahmed and Aleem Ahmed Khan and Nooruddin Owaisi and Akash Jaiswal and Mazharuddin Ali Khan},

url = {https://onlinelibrary.wiley.com/doi/10.1111/iji.12366},

doi = {10.1111/iji.12366},

issn = {1744-3121},

year  = {2018},

date = {2018-06-04},

urldate = {2018-06-01},

journal = {International Journal of Immunogenetics},

volume = {45},

issue = {4},

pages = {190-200},

publisher = {Wiley},

abstract = {This study aimed to investigate the genetic basis of ankylosing spondylitis (AS) and polyarthralgia (PA) conditions among Indian subjects through genotyping two immune regulatory genes CD14 (−159C>T) and MIF (−173G>C) and find their association with the expression levels of three circulating inflammatory miRNAs. This investigation may provide early genetic cause of these two forms of arthritis and more optimal biological targets to predict early therapeutic outcomes. A total of 140 patients (AS: 70 and PA: 70) and 156 controls were recruited from Indian population. CD14 and MIF genotyping was performed using ARMS–PCR. Expression level of three inflammatory miRNAs (miRNA-146a, miRNA-155 and miRNA-181) was quantified using RT–qPCR. C/T genotype of CD14 gene was found to cause 2.06-fold risk of developing AS (CI 1.06–5.98},

key = {pmid29863307},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Khan_2018g,

title = {In vitro hemocompatability evaluation of gold nanoparticles capped with Lactobacillus plantarum derived lipase1},

author = {Imran Khan and Sandeep Kumar Vishwakarma and Aleem Ahmed Khan and Ganesan Ramakrishnan and Jayati Ray Dutta},

url = {https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch189117},

doi = {10.3233/ch-189117},

issn = {1386-0291},

year  = {2018},

date = {2018-05-04},

urldate = {2018-05-04},

journal = {Clinical Hemorheology and Microcirculation},

volume = {69},

issue = {1-2},

pages = {197-205},

publisher = {IOS Press},

abstract = {BACKGROUND:Gold nanoparticles (GNPs) are key diagnostic and therapeutic agents in biomedical sciences. Several studies have been carried out in different therapeutic areas such as in cancer treatment, antibacterial topical agents, imaging agents etc. There is a necessity to evaluate the gold nanoparticles cytotoxicity at all fronts. Since blood is the first point of contact in any therapy, it is required to have a thorough in vitro investigation of gold nanoparticles to avoid any adverse effects. OBJECTIVE:The objective of the current study is to evaluate the effect of gold nanoparticles capped with lipase on blood clotting factors, platelets, coagulation time and blood clotting strength. METHODS:Whole blood samples were drawn from healthy volunteers. Plasma and plasma with platelets were isolated from the blood and all the samples were treated with lipase capped gold nanoparticles, except control. Plasma fibrinogen formed in the blood coagulation process after contacting with nanoparticles was quantitatively evaluated. In addition, platelet aggregation, blood clotting kinetics, strength of the blood clot and time were evaluated post nanoparticle treatment. RESULTS:The work primarily explores the effect of GNPs on blood with changing concentrations of lipase capping. Plasma fibrinogen levels of plasma samples were found to be moderately elevated, however, there is no significant effect on blood clotting kinetics, strength, and platelet aggregation. Also, the study showed that lipase capped GNPs did not result in aggregation upon interaction with plasma components and remained stable for 1 hour after incubation. CONCLUSIONS:Our study revealed that lipase capped GNPs synthesized using NaBH4 approach were stable and hemocompatible. There is an increase in fibrinogen levels after the exposure to nanoparticles, an observation which is consistent with other studies. However, the functional consequences of such increase are unknown. The results of no significant platelet aggregation, change in blood clotting time, kinetics, and clot strength revealed the non-toxic effect of lipase capped GNPs towards blood components, which is essential for any in vivo applications.},

key = {pmid29630542},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Battu_2018,

title = {Amino acid starvation sensing dampens IL-1β production by activating riboclustering and autophagy},

author = {Srikanth Battu and Sumbul Afroz and Jeevan Giddaluru and Saima Naz and Weishan Huang and Saratchandra Singh Khumukcham and Rafiq Ahmad Khan and Saleem Yousuf Bhat and Insaf Ahmed Qureshi and Bramanandam Manavathi and Aleem Ahmed Khan and Avery August and Seyed Ehtesham Hasnain and Nooruddin Khan},

editor = {Douglas Green},

url = {https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.2005317&type=printable},

doi = {10.1371/journal.pbio.2005317},

issn = {1545-7885},

year  = {2018},

date = {2018-04-05},

urldate = {2018-04-05},

journal = {PLOS Biology},

volume = {16},

issue = {4},

pages = {e2005317},

publisher = {Public Library of Science (PLoS)},

abstract = {Activation of the amino acid starvation response (AAR) increases lifespan and acute stress resistance as well as regulates inflammation. However, the underlying mechanisms remain unclear. Here, we show that activation of AAR pharmacologically by Halofuginone (HF) significantly inhibits production of the proinflammatory cytokine interleukin 1β (IL-1β) and provides protection from intestinal inflammation in mice. HF inhibits IL-1β through general control nonderepressible 2 kinase (GCN2)–dependent activation of the cytoprotective integrated stress response (ISR) pathway, resulting in rerouting of IL-1β mRNA from translationally active polysomes to inactive ribocluster complexes—such as stress granules (SGs)—via recruitment of RNA-binding proteins (RBPs) T cell–restricted intracellular antigen-1(TIA-1)/TIA-1–related (TIAR), which are further cleared through induction of autophagy. GCN2 ablation resulted in reduced autophagy and SG formation, which is inversely correlated with IL-1β production. Furthermore, HF diminishes inflammasome activation through suppression of reactive oxygen species (ROS) production. Our study unveils a novel mechanism by which IL-1β is regulated by AAR and further suggests that administration of HF might offer an effective therapeutic intervention against inflammatory diseases.},

key = {pmid29621237},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2018f,

title = {Role of drug transporters and heat shock proteins during ethanol exposure to human neural precursor cells and its lineages},

author = {Sandeep Kumar Vishwakarma and Nusrath Fathima and Chandrakala Lakkireddy and Nagarapu Raju and Avinash Bardia and A. Sandhya and Syed Ameer Basha Paspala and Vishnupriya Satti and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0040816617302999?via%3Dihub},

doi = {10.1016/j.tice.2018.02.001},

issn = {0040-8166},

year  = {2018},

date = {2018-02-07},

urldate = {2018-04-01},

journal = {Tissue and Cell},

volume = {51},

pages = {14-23},

publisher = {Elsevier BV},

abstract = {Introduction: Ethanol exposure to developing brain may alter the growth and differentiation of neurological cells resulting in unfavorable pathologies. Earlier studies have provided very limited mechanistic insights of cellular and molecular mechanisms which do not mimic with human situation due to varying cell types and poses potential challenges for investigation. Therefore, the present study was undertaken to evaluate the role of ABC transporters and heat shock proteins mediated response in human neural precursor cells (NPCs) and its lineages during proliferation and lineage differentiation against ethanol exposure. Methods: Effect of ethanol exposure was examined for neuronal cell survival and variation in cellular phenotype during neurospheres development and lineage differentiation. Generation of reactive oxygen species, and variation in cell cycle was identified along with transcriptional profiling for pluripotent markers (Nestin, NCAM, Sox-2, and Notch-2), drug transporters (ABCB1 and ABCG2) and stress protein (HSP70) during ethanol exposure. Results: ABC transporters as well as HSP70 mRNA expression was higher during proliferation as compared to differentiation with chronic ethanol (1 M) exposure (p < 0.01). Ethanol exposure resulted in higher variability in size and shape of developing neurospheres and decreased ability to form new neurosphere colonies. Significant changes were observed in dendrite development due to late ethanol exposure (p < 0.0001). Conclusion: The present study demonstrated significant role of ABC transporters and HSP70 proteins in providing defense against ethanol-induced damage in human neurological cells. However, the over-expression of ABC transporter and HSP-70 proteins during such pathological conditions do not provide complete defense and additional strategies are required to repair the damage.},

key = {pmid29622083},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2018e,

title = {Bioengineered humanized livers as better three-dimensional drug testing model system},

author = {Sandeep Kumar Vishwakarma and Avinash Bardia and Chandrakala Lakkireddy and Raju Nagarapu and Md. Aejaz Habeeb and Aleem Ahmed Khan},

url = {https://f6publishing.blob.core.windows.net/50475439-e1e8-4d32-b8ca-07dbaee5e315/WJH-10-22.pdf},

doi = {10.4254/wjh.v10.i1.22},

issn = {1948-5182},

year  = {2018},

date = {2018-01-27},

urldate = {2018-01-01},

journal = {World Journal of Hepatology},

volume = {10},

issue = {1},

pages = {22-33},

publisher = {Baishideng Publishing Group Inc.},

abstract = {AIM: To develop appropriate humanized three-dimensional ex-vivo model system for drug testing. METHODS: Bioengineered humanized livers were developed in this study using human hepatic stem cells repopulation within the acellularized liver scaffolds which mimics with the natural organ anatomy and physiology. Six cytochrome P-450 probes were used to enable efficient identification of drug metabolism in bioengineered humanized livers. The drug metabolism study in bioengineered livers was evaluated to identify the absorption, distribution, metabolism, excretion and toxicity responses. RESULTS: The bioengineered humanized livers showed cellular and molecular characteristics of human livers. The bioengineered liver showed three-dimensional natural architecture with intact vasculature and extra-cellular matrix. Human hepatic cells were engrafted similar to the human liver. Drug metabolism studies provided a suitable platform alternative to available ex-vivo and in vivo models for identifying cellular and molecular dynamics of pharmacological drugs. CONCLUSION: The present study paves a way towards the development of suitable humanized preclinical model systems for pharmacological testing. This approach may reduce the cost and time duration of preclinical drug testing and further overcomes on the anatomical and physiological variations in xenogeneic systems.},

key = {pmid29399275},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2017c,

title = {Protective role of hypothermia against heat stress in differentiated and undifferentiated human neural precursor cells: a differential approach for the treatment of traumatic brain injury},

author = {Sandeep Kumar Vishwakarma and Avinash Bardia and Nusrath Fathima and Chandrakala Lakkireddy and Syed Rahamathulla and Nagarapu Raju and Gunda Srinivas and Avinash Raj and Annamaneni Sandhya and Vishnupriya Satti and Santosh Kumar Tiwari and Syed Ameer Basha Paspala and Aleem Ahmed Khan},

url = {https://bcn.iums.ac.ir/article-1-647-en.pdf},

doi = {10.29252/nirp.bcn.8.6.453},

issn = {2008-126X},

year  = {2017},

date = {2017-11-01},

urldate = {2017-11-01},

journal = {Basic and Clinical Neuroscience Journal},

volume = {8},

issue = {6},

pages = {453-466},

publisher = {Negah Scientific Publisher},

abstract = {Introduction: The present study aimed to explore protective mechanisms of hypothermia against mild cold and heat stress on highly proliferative homogeneous human Neural Precursor Cells (NPCs) derived from Subventricular Zone (SVZ) of human fetal brain. Methods: CD133+ve enriched undifferentiated and differentiated human NPCs were exposed to heat stress at 42°C. Then, Western-blot quantification was performed using Hsp-70 (70 kilodalton heat shock proteins) recombinant protein. Finally, changes in pluripotency and Hsp-70 expression were measured using immunofluorescence staining and RT-qPCR (Quantitative reverse transcription PCR) analysis, respectively. Results: Heat stress resulted in abnormal neurospheres development. The apoptosis rate was enhanced during long-term in vitro culture of neurospheres. Neurogenic differentiation reduced and showed aberrent phenotypes during heat stress. After hypothermia treatment significant improvement in neurospheres and neuronal cell morphology was observed. Conclusion: Mild-hypothermia treatment induces attenuated heat shock response against heat stress resulting in induced HSP-70 expression that significantly improves structure and function of both undifferentiated human NPCs and differentiated neurons.},

key = {pmid29942429},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2017b,

title = {Enhanced neuroprotective effect of mild-hypothermia with VPA against ethanol–mediated neuronal injury},

author = {Sandeep Kumar Vishwakarma and Avinash Bardia and Chandrakala Lakkireddy and Sana Arshiya and Syed Ameer Basha Paspala and Vishnupriya Satti and Aleem Ahmed Khan},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0040816617301374?via%3Dihub},

doi = {10.1016/j.tice.2017.09.004},

issn = {0040-8166},

year  = {2017},

date = {2017-09-14},

urldate = {2017-12-01},

journal = {Tissue and Cell},

volume = {49},

issue = {6},

pages = {638-647},

publisher = {Elsevier BV},

abstract = {Introduction: Progress in understanding pathophysiological mechanisms and the development of targeted regenerative strategies have been hampered by the lack of predictive disease models, specifically for the conditions to which affected cell types are inaccessible. The present study has aimed to unearth the role of valproic acid (VPA) and mild hypothermia (MH) as promising strategy to enhance the neuroprotective mechanisms in undifferentiated and differentiated human neural precursor cells (hNPCs) against ethanol-induced damage. Methods: 5 mM VPA alone or in combination with MH (33 °C) was used to prevent the damage in proliferating and differentiating hNPCs. CD133 + ve enriched hNPCs were cultured in vitro and exposed to 1 M chronic ethanol concentration for 72 h and followed by VPA and MH treatment for 24 h. Morphometric analysis was performed to identify changes in neurospheres development and neuronal cell phenotypes. Flow cytometry and RT-qPCR analysis was performed to investigate alterations in key molecular pathways involved in cell survival and signaling. Results: Combination of VPA with MH displayed higher proportion of neuronal cell viability as compared to single treatment. Combination treatment was most effective in reducing apoptosis and reactive oxygen species levels in both the undifferentiated and differentiated hNPCs. VPA with MH significantly improved neuronal cell phenotype, active chromatin modeling, chaperon and multi-drug resistant pumps activity and expression of neuronal signaling molecules. Conclusion: The study provided an efficient and disease specific in vitro model and demonstrated that combined treatment with VPA and MH activates several neuroprotective mechanisms and provides enhanced protection against ethanol-induced damage in cultured undifferentiated and differentiated hNPCs.},

key = {pmid28947065},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vishwakarma_2017,

title = {Use of biocompatible sorafenib-gold nanoconjugates for reversal of drug resistance in human hepatoblatoma cells},

author = {Sandeep Kumar Vishwakarma and Priyanka Sharmila and Avinash Bardia and Lakkireddy Chandrakala and N. Raju and G. Sravani and B. V. S. Sastry and Md. Aejaz Habeeb and Aleem Ahmed Khan and Marshal Dhayal},

url = {https://www.nature.com/articles/s41598-017-08878-y.pdf},

doi = {10.1038/s41598-017-08878-y},

issn = {2045-2322},

year  = {2017},

date = {2017-08-17},

urldate = {2017-08-01},

journal = {Scientific Reports},

volume = {7},

issue = {1},

pages = {1-12},

publisher = {Springer Science and Business Media LLC},

abstract = {The present study identifies the potential of highly biocompatible SF-GNP nano-conjugate to enhance the chemotherapeutic response to combat drug resistance in cancer cells. We developed a stable colloidal suspension of sorafenib-gold nanoconjugate (SF-GNP) of <10 nm size in aqueous medium for reverting the cancer drug resistance in SF-resistant HepG2 cells in a 3D ex-vivo model system. In-vivo biocompatibility assay of SF-GNPs showed absence of systemic toxicological effects including hematological, biochemical and histological parameters. More importantly, the histopathological analysis of vital organs such as liver, brain, lung, kidney and heart showed very least or no sign of inflammation, cell infiltration, necrosis, tissue disorganization or fibrotic reactions after intra-peritoneal administration of SF-GNP nanoconjugates in animals. However, SF-GNP nanoconjugates significantly reduced (>80%) the percentage cell survival and the size and number of SF resistant solid tumor colonies of HepG2 cells in 3D model system. The exposure of SF-GNP nanoconjugate to SF resistant HepG2 cell colonies also provided evidence for anti-proliferative effect and reversal of drug resistance by elucidating the molecular regulatory mechanisms of extracellular matrix factor (CD147), tumor growth factor (TGF-β), hepatoma upregulated protein (hURP) and drug transporter (ABCG-2).},

key = {pmid28819176},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kema_2017,

title = {Protective effects of diallyl sulfide against ethanol-induced injury in rat adipose tissue and primary human adipocytes},

author = {Venkata Harini Kema and Imran Khan and Reshma Jamal and Sandeep Kumar Vishwakarma and Chandrakala Lakkireddy and Kirti Parwani and Farhin Patel and Dhara Patel and Aleem Ahmed Khan and Palash Mandal},

url = {https://onlinelibrary.wiley.com/doi/10.1111/acer.13398},

doi = {10.1111/acer.13398},

issn = {2993-7175},

year  = {2017},

date = {2017-04-17},

urldate = {2017-05-01},

journal = {Alcoholism: Clinical and Experimental Research},

volume = {41},

issue = {6},

pages = {1078-1092},

publisher = {Wiley},

abstract = {Background: Alcohol consumption is the fourth leading cause of death and disability worldwide. Several cellular pathways contribute to alcohol-mediated tissue injury. Adipose tissue apart from functioning as an endocrine organ secretes several hormones and cytokines known as adipokines that are known to play a significant role in alcohol-induced tissue damage. This study was designed to test the efficacy of diallyl sulfide (DAS) in regulating the alcohol-induced outcomes on adipose tissue. Methods: Male Wistar rats were fed with 36% Lieber–DeCarli liquid diet containing ethanol (EtOH) for 4 weeks. Control rats were pair-fed with isocaloric diet containing maltodextrin instead of EtOH. During the last week of feeding protocol, the EtOH-fed rat group was given 200 mg/kg body weight of DAS through diet. We also studied DAS effect on isolated human primary adipocytes. Viability of human primary adipocytes on DAS treatment was assessed by MTT assay. Malondialdehyde (MDA), a marker of oxidative stress, was measured by HPLC and the thiobarbituric acid method. Expression of inflammatory genes and lipogenic genes was studied by qRT-PCR and Western blotting. Serum inflammatory gene expression was studied by ELISA. Results: Our study results showed that DAS could alleviate EtOH-induced expression levels of proinflammatory and endoplasmic reticulum (ER) stress genes and improve adipose tissue mass and adipocyte morphology in male Wistar rats fed Lieber–DeCarli diet containing 6% EtOH. Further, we showed that DAS reduced the expression of lipogenic genes and improved lipid accumulation and adipocyte mass in human primary adipocytes treated with EtOH. Subsequently, we also showed that oxidative stress, as measured by the changes in MDA levels, was reduced in both male Wistar rats and human primary adipocytes treated with EtOH plus DAS. Conclusions: Our study results prove that DAS is effective in ameliorating EtOH-induced damage to adipose tissue as evidenced by the reduction brought about by DAS in oxidative stress, ER stress, and proinflammatory gene expression levels. DAS treatment also regulated lipogenic gene expression levels, thereby reducing free fatty acid release. In conclusion, this study has clinical implications with respect to alcohol-induced adipose tissue injury among alcohol users.},

key = {pmid28414868},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Afroz_2017,

title = {A comprehensive gene expression meta-analysis identifies novel immune signatures in rheumatoid arthritis patients},

author = {Sumbul Afroz and Jeevan Giddaluru and Sandeep Vishwakarma and Saima Naz and Aleem Ahmed Khan and Nooruddin Khan},

url = {https://www.frontiersin.org/articles/10.3389/fimmu.2017.00074/full},

doi = {10.3389/fimmu.2017.00074},

issn = {1664-3224},

year  = {2017},

date = {2017-02-02},

urldate = {2017-02-01},

journal = {Frontiers in Immunology},

volume = {8},

pages = {1-12},

publisher = {Frontiers Media SA},

abstract = {Rheumatoid arthritis (RA), a symmetric polyarticular arthritis, has long been feared as one of the most disabling forms of arthritis. Identification of gene signatures associated with RA onset and progression would lead toward development of novel diagnostics and therapeutic interventions. This study was undertaken to identify unique gene signatures of RA patients through large-scale meta-profiling of a diverse collection of gene expression data sets. We carried out a meta-analysis of 8 publicly available RA patients’ (107 RA patients and 76 healthy controls) gene expression data sets and further validated a few meta-signatures in RA patients through quantitative real-time PCR (RT-qPCR). We identified a robust meta-profile comprising 33 differentially expressed genes, which were consistently and significantly expressed across all the data sets. Our meta-analysis unearthed upregulation of a few novel gene signatures including PLCG2, HLA-DOB, HLA-F, EIF4E2, and CYFIP2, which were validated in peripheral blood mononuclear cell samples of RA patients. Further, functional and pathway enrichment analysis reveals perturbation of several meta-genes involved in signaling pathways pertaining to inflammation, antigen presentation, hypoxia, and apoptosis during RA. Additionally, PLCG2 (phospholipase Cγ2) popped out as a novel meta-gene involved in most of the pathways relevant to RA including inflammasome activation, platelet aggregation, and activation, thereby suggesting PLCG2 as a potential therapeutic target for controlling excessive inflammation during RA. In conclusion, these findings highlight the utility of meta-analysis approach in identifying novel gene signatures that might provide mechanistic insights into disease onset, progression and possibly lead toward the development of better diagnostic and therapeutic interventions against RA.},

key = {pmid28210261},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Khan_2017e,

title = {Immunophenotypic and molecular analysis of human dental pulp stem cells potential for neurogenic differentiation},

author = {Aleem Ahmed Khan and Nikhat Fatima and Sandeep Kumar Vishwakarma},

url = {https://journals.lww.com/cocd/fulltext/2017/08010/immunophenotypic_and_molecular_analysis_of_human.16.aspx},

doi = {10.4103/ccd.ccd_998_16},

issn = {0976-2361},

year  = {2017},

date = {2017-01-01},

urldate = {2017-01-01},

journal = {Contemporary Clinical Dentistry},

volume = {8},

issue = {1},

pages = {81-89},

publisher = {Medknow},

abstract = {Background: Growing evidence shows that dental pulp (DP) tissues could be a potential source of adult stem cells for the treatment of devastating neurological diseases and several other conditions. Aims: Exploration of the expression profile of several key molecular markers to evaluate the molecular dynamics in undifferentiated and differentiated DP-derived stem cells (DPSCs) in vitro. Settings and Design: The characteristics and multilineage differentiation ability of DPSCs were determined by cellular and molecular kinetics. DPSCs were further induced to form adherent (ADH) and non-ADH (NADH) neurospheres under serum-free condition which was further induced into neurogenic lineage cells and characterized for their molecular and cellular diversity at each stage. Statistical Analysis Used: Statistical analysis used one-way analysis of variance, Student's t-test, Livak method for relative quantification, and R programming. Results: Immunophenotypic analysis of DPSCs revealed >80% cells positive for mesenchymal markers CD90 and CD105, >70% positive for transferring receptor (CD71), and >30% for chemotactic factor (CXCR3). These cells showed mesodermal differentiation also and confirmed by specific staining and molecular analysis. Activation of neuronal lineage markers and neurogenic growth factors was observed during lineage differentiation of cells derived from NADH and ADH spheroids. Greater than 80% of cells were found to express β-tubulin III in both differentiation conditions. Conclusions: The present study reported a cascade of immunophenotypic and molecular markers to characterize neurogenic differentiation of DPSCs under serum-free condition. These findings trigger the future analyses for clinical applicability of DP-derived cells in regenerative applications.},

key = {pmid28566856},

keywords = {},

pubstate = {published},

tppubtype = {article}

}